Screening of cytotoxic, anti-angiogenic, anti-tumorogenic and antimicrobial activities of anatolian vipera ammodytes (Nose-horned viper) venom [Anadolu’da yayılış gösteren vipera ammodytes (Burunlu engerek) zehrinin sitotoksik, antianjiyogenik, anti-tümör ve antimikrobiyal aktivitelerinin taranması]

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dc.contributor.authorİğci N.
dc.contributor.authorNalbantsoy A.
dc.contributor.authorErkan L.G.
dc.contributor.authorAkça G.Y.
dc.contributor.authorYalçın H.T.
dc.contributor.authorYalçın M.
dc.contributor.authorGöçmen B.
dc.date.accessioned2019-10-27T08:20:23Z
dc.date.available2019-10-27T08:20:23Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: In the present study, we aimed to screen the cytotoxic, antimicrobial, anti-angiogenic and anti-tumorogenic activities of Anatolian Vipera ammodytes (Nosehorned Viper) crude venom. Material and methods: The cytotoxicity was screened against PC3, HeLa, CaCo-2, U-87MG, MCF-7 and Vero cells by using MTT assay. The antimicrobial activity on Escherichia coli ATCC 25922, E. coli 0157:H7, Enterococcus faecalis 29212, Enterococcus faecium DSM 13590, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Salmonella typhimirium CCM 5445, Proteus vulgaris ATCC 6957, Bacillus cereus ATCC 7064 and Candida albicans ATCC 10239 was assayed by determining the minimum inhibitory concentration using the broth dilution method. Anti-angiogenic and anti-tumorogenic activity was assessed by using chick chorioallantoic membrane (CAM) assay. Results: The IC50 value of V. ammodytes venom on cultured cells varied from 1.8 to 7.0 µg/mL after 48 h treatment. Venom showed antimicrobial activity on P. vulgaris, S. aureus, S. epidermidis, E. faecium and C. albicans (the highest activity). The venom exhibited dose-dependent anti-angiogenic activity on CAM model at 2 and 10 µg/mL doses with scores of 1.1 and 2.0, respectively. Conclusion: The results of the present study contributed to the knowledge of the biological activities of Anatolian V. ammodytes venom and showed its potential for further bioactivity guided characterization studies. © 2016 Turkish Biochemistry Society. All rights reserved.en_US
dc.identifier.doi10.1515/tjb-2016-0195
dc.identifier.endpage491en_US
dc.identifier.issn0250-4685
dc.identifier.issn0250-4685en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage483en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0195
dc.identifier.urihttps://hdl.handle.net/11454/25631
dc.identifier.volume41en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkish Biochemistry Societyen_US
dc.relation.ispartofTurkish Journal of Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-angiogenic effecten_US
dc.subjectAntimicrobial activityen_US
dc.subjectCytotoxicityen_US
dc.subjectSnake venomen_US
dc.subjectVipera ammodytesen_US
dc.titleScreening of cytotoxic, anti-angiogenic, anti-tumorogenic and antimicrobial activities of anatolian vipera ammodytes (Nose-horned viper) venom [Anadolu’da yayılış gösteren vipera ammodytes (Burunlu engerek) zehrinin sitotoksik, antianjiyogenik, anti-tümör ve antimikrobiyal aktivitelerinin taranması]en_US
dc.typeArticleen_US

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