Screening of cytotoxic, anti-angiogenic, anti-tumorogenic and antimicrobial activities of anatolian vipera ammodytes (Nose-horned viper) venom [Anadolu’da yayılış gösteren vipera ammodytes (Burunlu engerek) zehrinin sitotoksik, antianjiyogenik, anti-tümör ve antimikrobiyal aktivitelerinin taranması]
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Biochemistry Society
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: In the present study, we aimed to screen the cytotoxic, antimicrobial, anti-angiogenic and anti-tumorogenic activities of Anatolian Vipera ammodytes (Nosehorned Viper) crude venom. Material and methods: The cytotoxicity was screened against PC3, HeLa, CaCo-2, U-87MG, MCF-7 and Vero cells by using MTT assay. The antimicrobial activity on Escherichia coli ATCC 25922, E. coli 0157:H7, Enterococcus faecalis 29212, Enterococcus faecium DSM 13590, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Salmonella typhimirium CCM 5445, Proteus vulgaris ATCC 6957, Bacillus cereus ATCC 7064 and Candida albicans ATCC 10239 was assayed by determining the minimum inhibitory concentration using the broth dilution method. Anti-angiogenic and anti-tumorogenic activity was assessed by using chick chorioallantoic membrane (CAM) assay. Results: The IC50 value of V. ammodytes venom on cultured cells varied from 1.8 to 7.0 µg/mL after 48 h treatment. Venom showed antimicrobial activity on P. vulgaris, S. aureus, S. epidermidis, E. faecium and C. albicans (the highest activity). The venom exhibited dose-dependent anti-angiogenic activity on CAM model at 2 and 10 µg/mL doses with scores of 1.1 and 2.0, respectively. Conclusion: The results of the present study contributed to the knowledge of the biological activities of Anatolian V. ammodytes venom and showed its potential for further bioactivity guided characterization studies. © 2016 Turkish Biochemistry Society. All rights reserved.
Açıklama
Anahtar Kelimeler
Anti-angiogenic effect, Antimicrobial activity, Cytotoxicity, Snake venom, Vipera ammodytes
Kaynak
Turkish Journal of Biochemistry
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
41
Sayı
6
