Evaluation of molecular characterization and phylogeny for quantification of Acanthamoeba and Naegleria fowleri in various water sources, Turkey

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dc.authoridAykur, Mehmet/0000-0002-6100-1037
dc.authorscopusid55334665700
dc.authorscopusid6602339839
dc.authorwosidAykur, Mehmet/I-2963-2012
dc.contributor.authorAykur, Mehmet
dc.contributor.authorDagci, Hande
dc.date.accessioned2023-01-12T20:10:45Z
dc.date.available2023-01-12T20:10:45Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractFree-living amoeba (FLA) is widely distributed in the natural environment. Since these amoebae are widely found in various waters, they pose an important public health problem. The aim of this study was to detect the presence of Acanthamoeba, B. mandrillaris, and N. fowleri in various water resources by qPCR in Izmir, Turkey. A total of (n = 27) 18.24% Acanthamoeba and (n = 4) 2.7% N. fowleri positives were detected in six different water sources using qPCR with ITS regions (ITS1) specific primers. The resulting concentrations varied in various water samples for Acanthamoeba in the range of 3.2x10(5)-1.4x10(2) plasmid copies/l and for N. fowleri in the range of 8x10(3)-11x10(2) plasmid copies/l. The highest concentration of Acanthamoeba and N. fowleri was found in seawater and damp samples respectively. All 27 Acanthamoeba isolates were identified in genotype level based on the 18S rRNA gene as T4 (51.85%), T5 (22.22%), T2 (14.81%) and T15 (11.11%). The four positive N. fowleri isolate was confirmed by sequencing the ITS1, ITS2 and 5.8S rRNA regions using specific primers. Four N. fowleri isolates were genotyped (three isolate as type 2 and one isolate as type 5) and detected for the first time from water sources in Turkey. Acanthamoeba and N. fowleri genotypes found in many natural environments are straightly related to human populations to have pathogenic potentials that may pose a risk to human health. Public health professionals should raise awareness on this issue, and public awareness education should be provided by the assistance of civil authorities. To the best of our knowledge, this is the first study on the quantitative detection and distribution of Acanthamoeba and N. fowleri genotypes in various water sources in Turkey.en_US
dc.description.sponsorshipCouncil of Higher Education; Scientific Research Projects Branch Directorate of Ege University, Turkey [18-TIP-025]en_US
dc.description.sponsorshipThe research was supported by a grant from the Budget of the Academic Staff Training Program by The Council of Higher Education and the Scientific Research Projects Branch Directorate of Ege University, Turkey (Project No: 18-TIP-025). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.identifier.doi10.1371/journal.pone.0256659
dc.identifier.issn1932-6203
dc.identifier.issue8en_US
dc.identifier.pmid34437614en_US
dc.identifier.scopus2-s2.0-85113814963en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0256659
dc.identifier.urihttps://hdl.handle.net/11454/77913
dc.identifier.volume16en_US
dc.identifier.wosWOS:000697186000076en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFree-Living Amebasen_US
dc.subjectRibosomal-Rna Geneen_US
dc.subjectInternal Transcribed Spacersen_US
dc.subjectTime Pcr Methodsen_US
dc.subjectPathogenic Acanthamoebaen_US
dc.subjectBalamuthia-Mandrillarisen_US
dc.subjectT7 Genotypesen_US
dc.subjectTap Wateren_US
dc.subjectIdentificationen_US
dc.subjectKeratitisen_US
dc.titleEvaluation of molecular characterization and phylogeny for quantification of Acanthamoeba and Naegleria fowleri in various water sources, Turkeyen_US
dc.typeArticleen_US

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