Development of living cell microarrays using non-contact micropipette printing

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dc.contributor.authorJonczyk, Rebecca
dc.contributor.authorTimur, Suna
dc.contributor.authorScheper, Thomas
dc.contributor.authorStahl, Frank
dc.date.accessioned2019-10-27T23:11:48Z
dc.date.available2019-10-27T23:11:48Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractDuring the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contactfree spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue (R) Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation: +/- 8.6 cells) printing method for small living cell amounts (1200 cells and fewer) was established that achieved cell viabilities of up to 88% for >0.6 mu L and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipFederal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF); Niedersachsische Krebsgesellschaft e.V.en_US
dc.description.sponsorshipThis work was supported by Federal Ministry of Education and Research (BMBF) and by Niedersachsische Krebsgesellschaft e.V. We are grateful to Dr. Antonina Lavrentieva and Dr. Johanna Walter for her critical reading of the manuscript. We further thank Paul Maschhoff for proofreading of the manuscript.en_US
dc.identifier.doi10.1016/j.jbiotec.2015.11.013en_US
dc.identifier.endpage111en_US
dc.identifier.issn0168-1656
dc.identifier.issn1873-4863
dc.identifier.pmid26603124en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiotec.2015.11.013
dc.identifier.urihttps://hdl.handle.net/11454/53130
dc.identifier.volume217en_US
dc.identifier.wosWOS:000366757900015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicroarray technologyen_US
dc.subjectPiezoelectric nanoprinteren_US
dc.subjectLiving mammalian cellen_US
dc.titleDevelopment of living cell microarrays using non-contact micropipette printingen_US
dc.typeArticleen_US

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