Solanaceae familyasındaki bazı kültür bitkilerinde potato virus Y (PVY)'nin ırklarının belirlenmesi ve karakterizasyonu
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi, Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Solanaceae bitkilerinin yoğun ve polikültür olarak
yetiştiriciliğinin yapıldığı İzmir ili Küçük Menderes Havzası’ndan toplanan
patates, domates ve biber bitkilerinde PVY ırklarının bulunma durumu,
karakterizasyonu, afitlerle taşınma durumunun moleküler olarak belirlenmesi,
bölgede yoğun olarak yetiştirilen çeşitlerin bu ırklara verdiği reaksiyonların
belirlenmesi ve patates, biber çeşitlerinin bu ırklara dayanıklılık durumlarının
markörlerle araştırılması amaçlanmıştır. Sörveylerden toplanan ve serolojik,
moleküler yöntemlerle test edilen, örneklerin %58,78’i çeşitli PVY ırklarıyla
enfekteli bulunmuştur. Patateste PVYN:O, domateste PVYNTN ve biberde PVYC en
yaygın görülen ırklar olarak belirlenmiştir. PVY ırklarının filogenetik analizi
sonucunda birbirine en benzer iki izolat PVYNTN/Patates ve PVYNTN/Domates izolatları
olmuştur. PVYNTN/Domates ve PVYN:O/Patates izolatlarının HC-Pro bölgelerinin
moleküler açıdan incelenmesi sonucu her iki izolatın da afitlerle taşınma
yeteneğinde olduğu belirlenmiştir. Çeşit reaksiyon denemesi sonuçlarına göre
patates çeşitlerinden Agria, Marabel, Bahar çeşitleri PVYN:O ve PVYNTN
enfeksiyonlarından; domates çeşitlerinden Albeni ve Advance PVYN:O, Albeni,
Advance, CXD 263 ve Zeplin çeşitleri PVYNTN enfeksiyonlarından çeşitli
oranlarda etkilenmiştir. Ergenekon, Yalova Yağlık 28, Deren ve Alexus biber
çeşitleri PVYC enfeksiyona değişik düzeylerde reaksiyon vermiş, Ergenekon çeşidi
hastalık şiddetinin en yüksek seyrettiği çeşit olmuştur. PVY dayanıklılığının
belirlenmesi için yürütülen çalışmalarda patateste Ryadg genine bağımlı RYSC3
markörü başarılı olamazken, Alexus, Sağanak, Anemon ve Armada biber
çeşitlerinin PVYC’ye dayanıklı olduğu saptanmıştır.
In this study, the status of PVY strains in potato, tomato and pepper plants collected from İzmir Küçük Menderes Basin, where solanaceous plants are cultivated intensively and as polyculture, has been investigated. Also, It was aimed to characterize the PVY strains, determine the transmission status by aphids molecularly, identify the reactions of potato, tomato and pepper varieties which are intensely grown in the basin to these strains and investigate the resistance of potato and pepper varieties to these strains with molecular markers. 58,78% of the plant leaf samples were collected from the surveys and were tested by serological, molecular methods, were found to be infected with various PVY strains. The most common PVY strains were PVYN:O in potato leaf samples, PVYNTN in tomato leaf samples and PVYC in pepper samples. As a result of phylogenetic analysis of PVY strains detected in the solanaceous plants, the two most similar isolates were PVYNTN/Potato and PVYNTN/Tomato isolates. As a result of the analysis of the amino acid sequences of the partially amplified HC-Pro regions of the PVYNTN/Tomato and PVYN:O/Potato isolates, it was determined that both isolates were aphid transmissible. According to results of variety reaction experiments, Agria, Marabel and Bahar potato varieties from PVYN:O and PVYNTN infections; Albeni and Advance tomato varieties from PVYN:O infections; Albeni, Advance, CXD 263 and Zeplin tomato varieties from PVYNTN infections were affected in various rates. Ergenekon, Yalova Yağlık 28, Deren and Alexus pepper varieties have reacted to PVYC infections in different rates, Ergenekon variety has the highest disease severity. In studies which was determined of PVY resistant varieties, Alexus, Sağanak and Armada pepper varieties has been resistant to PVYC strain, while RYSC3 marker dependent on Ryadg resistance gene in patato has not been successful.
In this study, the status of PVY strains in potato, tomato and pepper plants collected from İzmir Küçük Menderes Basin, where solanaceous plants are cultivated intensively and as polyculture, has been investigated. Also, It was aimed to characterize the PVY strains, determine the transmission status by aphids molecularly, identify the reactions of potato, tomato and pepper varieties which are intensely grown in the basin to these strains and investigate the resistance of potato and pepper varieties to these strains with molecular markers. 58,78% of the plant leaf samples were collected from the surveys and were tested by serological, molecular methods, were found to be infected with various PVY strains. The most common PVY strains were PVYN:O in potato leaf samples, PVYNTN in tomato leaf samples and PVYC in pepper samples. As a result of phylogenetic analysis of PVY strains detected in the solanaceous plants, the two most similar isolates were PVYNTN/Potato and PVYNTN/Tomato isolates. As a result of the analysis of the amino acid sequences of the partially amplified HC-Pro regions of the PVYNTN/Tomato and PVYN:O/Potato isolates, it was determined that both isolates were aphid transmissible. According to results of variety reaction experiments, Agria, Marabel and Bahar potato varieties from PVYN:O and PVYNTN infections; Albeni and Advance tomato varieties from PVYN:O infections; Albeni, Advance, CXD 263 and Zeplin tomato varieties from PVYNTN infections were affected in various rates. Ergenekon, Yalova Yağlık 28, Deren and Alexus pepper varieties have reacted to PVYC infections in different rates, Ergenekon variety has the highest disease severity. In studies which was determined of PVY resistant varieties, Alexus, Sağanak and Armada pepper varieties has been resistant to PVYC strain, while RYSC3 marker dependent on Ryadg resistance gene in patato has not been successful.
Açıklama
Anahtar Kelimeler
Solanaceae Familyası, PVY Irkları, Marköre Dayalı Seleksiyon (MAS), Çeşit Reaksiyonu, HC-Pro, Solanaceae Family, PVY Strains, Marker Associated Selection (MAS), Variety Response, HC-Pro
