Biofunctionalization of PAMAM-montmorillonite decorated poly (epsilon-caprolactone)-chitosan electrospun nanofibers for cell adhesion and electrochemical cytosensing

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dc.contributor.authorKirbay, Fatma Ozturk
dc.contributor.authorYalcinkaya, Esra Evrim
dc.contributor.authorAtik, Gozde
dc.contributor.authorEvren, Gizem
dc.contributor.authorUnal, Betul
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T10:04:48Z
dc.date.available2019-10-27T10:04:48Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractThe construction and biofunctionalization of the poly (C-caprolactone) (PCL)-chitosan (CHIT) nanofibrous mats, which included Polyamidoamine (PAMAM) dendrimer modified montmorillonite (Mt), for the cell adhesion and electrochemical cytosensing were accomplished in this report. After the intercalation of the PAMAM generation zero dendrimer into the Mt, PAMAM-Mt decorated PCL-CHIT electrospun nanofibers were formed. The addition of PAMAM caused the decrease of contact angle of PCL-CHIT nanofibers. The covalent immobilization of a tripeptide namely Arginylglycylaspartate (RGD) on both the PCL-CHIT/Mt and PCL-CHIT/PAMAM-Mt surface was carried out. U87-MG and HaCaT (negative control) cell lines were incubated on the PCL-CHIT/Mt/RGD and PCL-CHIT/PAMAM-Mt/RGD. The proliferation studies and imaging of the cells were carried out on these fibers. Finally, electrochemical measurements were performed after each modification step by differential pulse/cyclic voltammetry and electrochemical impedance spectroscopy. U87-MG cells were grown better than HaCaT cells on the PCL-CHIT/PAMAM-Mt/RGD surfaces. To the best of our knowledge, there is no study that developed electrochemical cytosensor using electrospun nanofibers as a cell adhesion platform.en_US
dc.description.sponsorshipEge University Research FoundationEge University [15-FEN-057, 17-FEN-011]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215Z194, 2209A]; Turkish Academy of Sciences-Outstanding Young Scientists Award Programen_US
dc.description.sponsorshipThis project was supported by Ege University Research Foundation (grande number: 15-FEN-057 and 17-FEN-011), the Scientific and Technological Research Council of Turkey (TUBITAK, project no 215Z194 and 2209A) and Dr. D. O. DEMIRKOL thanks to Turkish Academy of Sciences-Outstanding Young Scientists Award Program-2015 for funding the research of Sciences-Outstanding Young Scientists Award Program-2015 (TUBA-GEBIP-2015) and Dr. Tamer Uyar for his helps to configure Electrospinning Unit.en_US
dc.identifier.doi10.1016/j.bios.2018.03.017en_US
dc.identifier.endpage294en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.pmid29573728en_US
dc.identifier.startpage286en_US
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.03.017
dc.identifier.urihttps://hdl.handle.net/11454/30284
dc.identifier.volume109en_US
dc.identifier.wosWOS:000430778400038en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Advanced Technologyen_US
dc.relation.ispartofBiosensors & Bioelectronicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrospun nanofiberen_US
dc.subjectPoly(epsilon-caprolactone)en_US
dc.subjectChitosanen_US
dc.subjectBiofunctional surfaceen_US
dc.subjectCyctosensingen_US
dc.subjectCell adhesionen_US
dc.titleBiofunctionalization of PAMAM-montmorillonite decorated poly (epsilon-caprolactone)-chitosan electrospun nanofibers for cell adhesion and electrochemical cytosensingen_US
dc.typeArticleen_US

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