Continuous Microfluidic Production of Citrem-Phosphatidylcholine Nano-Self-Assemblies for Thymoquinone Delivery

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dc.authoridYaghmur, Anan/0000-0003-1608-773X
dc.authoridIlhan Ayisigi, Esra/0000-0003-1880-4261
dc.authoridDimaki, Maria/0000-0001-5037-2388
dc.authoridYesil-Celiktas, Ozlem/0000-0003-4509-2212
dc.authoridSvendsen, Winnie Edith/0000-0002-9197-6505
dc.authorscopusid57200659470
dc.authorscopusid57191035594
dc.authorscopusid55984908600
dc.authorscopusid6506249082
dc.authorscopusid6701422500
dc.authorscopusid22837401200
dc.authorscopusid6602560099
dc.authorwosidYaghmur, Anan/AGU-9381-2022
dc.authorwosidYesil-Celiktas, Ozlem/AAI-5713-2020
dc.authorwosidSvendsen, Winnie Edith/A-2976-2012
dc.authorwosidDimaki, Maria/J-9985-2012
dc.contributor.authorIlhan-Ayisigi, Esra
dc.contributor.authorGhazal, Aghiad
dc.contributor.authorSartori, Barbara
dc.contributor.authorDimaki, Maria
dc.contributor.authorSvendsen, Winnie Edith
dc.contributor.authorYesil-Celiktas, Ozlem
dc.contributor.authorYaghmur, Anan
dc.date.accessioned2023-01-12T20:15:42Z
dc.date.available2023-01-12T20:15:42Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractLamellar and non-lamellar liquid crystalline nanodispersions, including liposomes, cubosomes, and hexosomes are attractive platforms for drug delivery, bio-imaging, and related pharmaceutical applications. As compared to liposomes, there is a modest number of reports on the continuous production of cubosomes and hexosomes. Using a binary lipid mixture of citrem and soy phosphatidylcholine (SPC), we describe the continuous production of nanocarriers for delivering thymoquinone (TQ, a substance with various therapeutic potentials) by employing a commercial microfluidic hydrodynamic flow-focusing chip. In this study, nanoparticle tracking analysis (NTA) and synchrotron small-angle X-ray scattering (SAXS) were employed to characterize TQ-free and TQ-loaded citrem/SPC nanodispersions. Microfluidic synthesis led to formation of TQ-free and TQ-loaded nanoparticles with mean sizes around 115 and 124 nm, and NTA findings indicated comparable nanoparticle size distributions in these nanodispersions. Despite the attractiveness of the microfluidic chip for continuous production of citrem/SPC nano-self-assemblies, it was not efficient as comparable mean nanoparticle sizes were obtained on employing a batch (discontinuous) method based on low-energy emulsification method. SAXS results indicated the formation of a biphasic feature of swollen lamellar (L-alpha) phase in coexistence with an inverse bicontinuous cubic Pn3m phase in all continuously produced TQ-free and TQ-loaded nanodispersions. Further, a set of SAXS experiments were conducted on samples prepared using the batch method for gaining further insight into the effects of ethanol and TQ concentration on the structural features of citrem/SPC nano-self-assemblies. We discuss these effects and comment on the need to introduce efficient microfluidic platforms for producing nanocarriers for delivering TQ and other therapeutic agents.en_US
dc.description.sponsorshipDanish Council for Independent Research| Technology and Production Sciences [DFF-7017-00065]; Danish Natural Sciences Research Council (DanScatt); CERIC-ERIC Consortiumen_US
dc.description.sponsorshipFinancial support by the Danish Council for Independent Research| Technology and Production Sciences, reference DFF-7017-00065 (to A.Y.) is gratefully acknowledged. A.Y. further acknowledges financial support from the Danish Natural Sciences Research Council (DanScatt) for SAXS experiments. The authors acknowledge the CERIC-ERIC Consortium for financial support to the PhD student EI-A.en_US
dc.identifier.doi10.3390/nano11061510
dc.identifier.issn2079-4991
dc.identifier.issue6en_US
dc.identifier.pmid34200457en_US
dc.identifier.scopus2-s2.0-85107309260en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/nano11061510
dc.identifier.urihttps://hdl.handle.net/11454/78553
dc.identifier.volume11en_US
dc.identifier.wosWOS:000666468700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofNanomaterialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectthymoquinoneen_US
dc.subjectinverse bicontinuous cubic Pn3m phaseen_US
dc.subjectmicrofluidicsen_US
dc.subjectnanoparticle tracking analysisen_US
dc.subjectsynchrotron small-angle scatteringen_US
dc.subjectLiquid-Crystalline Particlesen_US
dc.subjectDrugen_US
dc.subjectCubosomesen_US
dc.subjectCarriersen_US
dc.subjectDispersionsen_US
dc.subjectHexosomesen_US
dc.subjectPhospholipidsen_US
dc.subjectLamellaren_US
dc.subjectSystemsen_US
dc.subjectDesignen_US
dc.titleContinuous Microfluidic Production of Citrem-Phosphatidylcholine Nano-Self-Assemblies for Thymoquinone Deliveryen_US
dc.typeArticleen_US

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