Determination of sand fly fauna and molecular detection of Leishmania in sand flies in Antalya Province, Southern Turkey

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dc.authoridCetin, Huseyin/0000-0002-9758-6356
dc.authoridARSERIM, SUHA KENAN/0000-0002-9174-6291
dc.authorscopusid55911250900
dc.authorscopusid57200807245
dc.authorscopusid55910580100
dc.authorscopusid35200249600
dc.authorscopusid55622378000
dc.authorscopusid55665073900
dc.authorscopusid55890674000
dc.authorwosidTöz, Seray/AHD-3178-2022
dc.authorwosidCetin, Huseyin/C-2242-2016
dc.contributor.authorArserim, Suha Kenan
dc.contributor.authorCetin, Huseyin
dc.contributor.authorKarakus, Mehmet
dc.contributor.authorDemir, Samiye
dc.contributor.authorSer, Onder
dc.contributor.authorToz, Seray
dc.contributor.authorBalcioglu, I. Cuneyt
dc.date.accessioned2023-01-12T19:50:13Z
dc.date.available2023-01-12T19:50:13Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractVisceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are diseases transmitted by infected female sand flies. Since the eradication of malaria in Turkey, CL is the main vector-borne disease in the country, with more than 2000 cases per year, making it a significant public health problem. The aims of this study were to carry out an entomological survey in Antalya Province, an endemic area for CL in the Mediterranean Region of Turkey, to identify sand fly fauna and to screen female specimens for the presence of Leishmania parasites (Leishmania infantum, L. tropica, L. major, and L. donovani) using molecular analysis. Sand flies were collected in 42 localities of seven districts in Antalya Province using CDC miniature light traps in two different periods, June 2012 and September 2013. The specimens were kept in 96% ethanol until the dissection was done. The head and genitalia of the specimens were cut for preparing individual slides for species identification. The rest of the body of female specimens was kept separately. The specimens were identified at the species level, and 27 pools were generated according to the locations and species for screening the presence of Leishmania. A commercial kit was used for DNA extractions. Real-time and conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region (ITS1) were then performed. In total, 1306 specimens comprising nine species belonging to the Phlebotomus genus were collected in the study region, with Phlebotomus neglectus/syriacus (38.82%) the most abundant, followed by P. alexandri (21.67%) and P. tobbi (20.44%). In the 27 pools, Leishmania infantum DNA was detected in four pools containing P. neglectus/syriacus and one pool containing P. tobbi. In conclusion, the sand fly fauna in the Antalya Province is diverse. The probable vector sand fly species are P. neglectus/syriacus and P. tobbi with high dominance (59.26%), which indicates a high risk of CL transmission. The data presented here may help to shed more light on the transmission cycles of the Leishmania parasite in this CL endemic area.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [112T270]en_US
dc.description.sponsorshipThis study is supported by The Scientific and Technological Research Council of Turkey (Project No. 112T270).en_US
dc.identifier.doi10.1007/s00436-021-07279-5
dc.identifier.endpage3111en_US
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955
dc.identifier.issue9en_US
dc.identifier.pmid34387751en_US
dc.identifier.scopus2-s2.0-85112417986en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage3105en_US
dc.identifier.urihttps://doi.org/10.1007/s00436-021-07279-5
dc.identifier.urihttps://hdl.handle.net/11454/76049
dc.identifier.volume120en_US
dc.identifier.wosWOS:000684756200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofParasitology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCutaneous leishmaniasisen_US
dc.subjectLeishmaniasisen_US
dc.subjectLeishmania infantumen_US
dc.subjectSand flyen_US
dc.subjectVectorsen_US
dc.subjectCutaneous Leishmaniasisen_US
dc.subjectPhlebotomine Sandfliesen_US
dc.subjectPsychodidaeen_US
dc.subjectDipteraen_US
dc.subjectNeglectusen_US
dc.titleDetermination of sand fly fauna and molecular detection of Leishmania in sand flies in Antalya Province, Southern Turkeyen_US
dc.typeArticleen_US

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