Bioassay-guided isolation of antibacterial and cytotoxic compounds from the mesophilic actinomycete M-33-5

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dc.contributor.authorUrgen M.
dc.contributor.authorKocabaş F.
dc.contributor.authorNalbantsoy A.
dc.contributor.authorKocabas E.H.
dc.contributor.authorUzel A.
dc.contributor.authorBedir E.
dc.date.accessioned2019-10-26T22:34:33Z
dc.date.available2019-10-26T22:34:33Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractOne hundred and twenty-six mesophilic Actinomycete cultures were isolated from the Aegean region of Turkey. The antimicrobial activities of pure isolates were tested using the agar-plaque method. Based on high antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) and Escherichia coli O157-H7 (E. coli), the isolate M-33-5 was selected for bioactivity-guided isolation. Fermentation, followed by solvent partition (H2O-EtOAc, H2O-n-BuOH), showed that the highest activity was present in the EtOAc extract. By using chromatographic methods, two bioactive compounds were isolated and their structures were determined by spectral methods to be 4'-deacetyl griseusin A and griseusin A. The MIC values of griseusin A and 4'-deacetyl griseusin A against MRSA and E. coli were ? 1.0 µg/mL. The cytotoxicities of the EtOAc extract and 4'-deacetyl griseusin A were also evaluated against two cancer cell lines (human servical cancer: HeLa; murine fibroblastic cells: L-929). The EtOAc extract showed strong cytotoxic activity against HeLa and L-929 lines with IC50 values of 1.57 and 2.43 µg/mL, respectively, whereas 4'-deacetyl griseusin A was very potent with IC50 values of 0.43 versus HeLa, and 0.12 µg/mL against L-929. The active strain M-33-5 was identified as Streptomyces griseus by 16SrDNA sequence data.en_US
dc.identifier.endpage252en_US
dc.identifier.issn1934-578X
dc.identifier.issue2en_US
dc.identifier.pmid20334137en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage249en_US
dc.identifier.urihttps://hdl.handle.net/11454/19818
dc.identifier.volume5en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofNatural Product Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject4'-deacetyl griseusin Aen_US
dc.subjectActinomyceteen_US
dc.subjectAntimicrobial activityen_US
dc.subjectCytotoxic activityen_US
dc.subjectEscherichia coli 232en_US
dc.subjectGriseusin Aen_US
dc.subjectMethicillin resistant Staphylococcus aureusen_US
dc.subjectStreptomyces griseusen_US
dc.titleBioassay-guided isolation of antibacterial and cytotoxic compounds from the mesophilic actinomycete M-33-5en_US
dc.typeArticleen_US

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