Selective Cell Adhesion and Biosensing Applications of Bio-Active Block Copolymers Prepared by CuAAC/Thiol-ene Double Click Reactions

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dc.contributor.authorOyman Eyrilmez G.
dc.contributor.authorDoran S.
dc.contributor.authorMurtezi E.
dc.contributor.authorDemir B.
dc.contributor.authorOdaci Demirkol D.
dc.contributor.authorCoskunol H.
dc.contributor.authorTimur S.
dc.contributor.authorYagci Y.
dc.date.accessioned2019-10-26T21:21:50Z
dc.date.available2019-10-26T21:21:50Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractN-Acetyl-l-cysteine (NAC)-capped poly(methyl methacrylate)-b-polycaprolactone block copolymer (PMMA-b-PCL-NAC) was prepared using the previously described one-pot photoinduced sequential CuAAC/thiol-ene double click procedure. PMMA-b-PCL-NAC had previously shown good applicability as a matrix for cell adhesion of cells from the Vero cell line (African green monkey kidney epithelial). Here, in this work, PMMA-b-PCL-NAC served as an excellent immobilization matrix for biomolecule conjugation. Covalent binding of RGD (R: arginine, G: glycine, and D: aspartic acid) peptide sequence onto the PMMA-b-PCL-NAC-coated surface was performed via EDC chemistry. RGD-modified PMMA-b-PCL-NAC (PMMA-b-PCL-NAC-RGD) as a non-toxic cell proliferation platform was used for selective "integrin ?vß3-mediated cell adhesion and biosensing studies. Both optical and electrochemical techniques were used to monitor the adhesion differences between "integrin ?vß3" receptor positive and negative cell lines on to the designed biofunctional surfaces. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.identifier.doi10.1002/mabi.201500099en_US
dc.identifier.endpage1241en_US
dc.identifier.issn1616-5187
dc.identifier.issue9en_US
dc.identifier.pmid25974890en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1233en_US
dc.identifier.urihttps://doi.org/10.1002/mabi.201500099
dc.identifier.urihttps://hdl.handle.net/11454/16913
dc.identifier.volume15en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-VCH Verlagen_US
dc.relation.ispartofMacromolecular Bioscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcell adhesionen_US
dc.subjectcell sensingen_US
dc.subjectelectrochemical biosensoren_US
dc.subjectselective surfacesen_US
dc.titleSelective Cell Adhesion and Biosensing Applications of Bio-Active Block Copolymers Prepared by CuAAC/Thiol-ene Double Click Reactionsen_US
dc.typeArticleen_US

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