Electrochemical biosensor for the interaction of DNA with the alkylating agent 4,4-dihydroxy chalcone based on guanine and adenine signals

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dc.contributor.authorMeric, B
dc.contributor.authorKerman, K
dc.contributor.authorOzkan, D
dc.contributor.authorKara, P
dc.contributor.authorErdem, A
dc.contributor.authorKucukoglu, O
dc.contributor.authorErciyas, E
dc.contributor.authorOzsoz, M
dc.date.accessioned2019-10-27T18:42:42Z
dc.date.available2019-10-27T18:42:42Z
dc.date.issued2002
dc.departmentEge Üniversitesien_US
dc.description.abstractThe interaction of an alkylating agent, 4,4'-dihydroxy chalcone (DHC) with calf thymus double stranded DNA (dsDNA) and calf thymus single stranded DNA (ssDNA) was studied electrochemically based on the oxidation signals of guanine and adenine by using differential pulse voltammetry (DPV) at carbon paste electrode (CPE). As a result of the alkylation of DHC between the base pairs in dsDNA, the voltammetric signal of guanine and adenine greatly decreased. After the interaction of DHC with ssDNA, a higher decrease in the oxidation signals of guanine and adenine was observed under the same conditions. The partition coefficients of DHC at dsDNA and ssDNA modified CPEs were calculated. The interactions of DHC with synthetic polynucleotides, such as polyguanylic acid and polyadenylic acid were also observed. In addition, the detection limit and the reproducibility were determined by using DPV. The interaction of DHC with dsDNA in solution-phase was also investigated and the results were compared with the ones obtained by surface immobilized dsDNA. The application of electrochemical DNA biosensor for monitoring the DNA-alkylating agent interactions was explored. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.doi10.1016/S0731-7085(02)00477-6en_US
dc.identifier.endpage1346en_US
dc.identifier.issn0731-7085
dc.identifier.issue4en_US
dc.identifier.pmid12408924en_US
dc.identifier.startpage1339en_US
dc.identifier.urihttps://doi.org/10.1016/S0731-7085(02)00477-6
dc.identifier.urihttps://hdl.handle.net/11454/37147
dc.identifier.volume30en_US
dc.identifier.wosWOS:000179506100046en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectelectrochemical DNA biosensoren_US
dc.subjectguanineen_US
dc.subjectadenineen_US
dc.subject4,4 '-dihydroxy chalconeen_US
dc.subjectDNAen_US
dc.titleElectrochemical biosensor for the interaction of DNA with the alkylating agent 4,4-dihydroxy chalcone based on guanine and adenine signalsen_US
dc.typeArticleen_US

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