Topraktan izole edilen bir aktinomiset straininin glukoz izomeraz üretimine ve aktivitesine etkili bazı faktörlerin saptanması
Küçük Resim Yok
Tarih
1997
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
16 farklı toprak örneğinden 139 Aktinomiset straini izole edilmiş, 104 strainin glukoz İzomeraz ürettiği saptanmış ve bunların arasından 1-8 nolu strainin en iyi GI üreticisi olarak belirlenmiştir. Bir Streptomyces sp. olarak tanımlanan bu strain, %1 ksiloz, %2 mısır ıslatma sıvısı, % 0.1 MgSC>4 7H2O ve % 0.01 C0CI2 6H2O içeren bir ortamda, 200 rpm* de, pH 7.0 de, 30 °C de, 24 saat büyütüldüğünde en yüksek glukoz izomeraz üretmiş; diğer taraftan 70 °C de, pH 7.0' da 1 saatlik inkübasyon sonunda en yüksek GI aktivitesi göstermiş; glukoz, Mg+2 ve Co+2 için Km değerleri 0.268 M, 60 mM ve 3.4 mM olarak saptanmıştır. 70 °C'de 84 saat yanlanma süresi olan bu enzimin oldukça termostabil olduğu görülmüştür. Anahtar Kelimeler: Glukoz İzomeraz, Tarama, Aktinomiset
Actinomycetes strains were isolated from the different soils; 104 of the 139 strains showed glucose isomerase activity. As 1-8 numbered strain was determined best glucose isomerase producer among the 104 strains. 1-8, identified as a Streptomyces sp, grown in the medium contained %1 xylose, %2 cornsteep liqueur, %0,1 MgS04-7H20 and %0,01 C0CI2 6H20, at pH 7.0 at 30°C and at speed of 200 rpm for 24 hours showed best production of glucose isomerase; on the other hand, this strain showed best activity at pH 7.0, at 70°C for an hour; the michealis constant for glucose, Mg2+ and Co2+ were found 0.268 M, 60 mM and 3.4 mM, respectively. This glucose isomerase was quite heat stable, with a half-life of 84 hours at 70°C. Keywords: Glucose Isomerase, Screening, Actinomycetes
Actinomycetes strains were isolated from the different soils; 104 of the 139 strains showed glucose isomerase activity. As 1-8 numbered strain was determined best glucose isomerase producer among the 104 strains. 1-8, identified as a Streptomyces sp, grown in the medium contained %1 xylose, %2 cornsteep liqueur, %0,1 MgS04-7H20 and %0,01 C0CI2 6H20, at pH 7.0 at 30°C and at speed of 200 rpm for 24 hours showed best production of glucose isomerase; on the other hand, this strain showed best activity at pH 7.0, at 70°C for an hour; the michealis constant for glucose, Mg2+ and Co2+ were found 0.268 M, 60 mM and 3.4 mM, respectively. This glucose isomerase was quite heat stable, with a half-life of 84 hours at 70°C. Keywords: Glucose Isomerase, Screening, Actinomycetes
Açıklama
Anahtar Kelimeler
Biyoloji, Biology, Aktinomisetler, Actinomycetes, Glükoz izomeraz, Glucose isomerase, İzomerazlar, Isomerases
