The Expressions of Small Ubiquitin-like Modifier (SUMO) Related Genes Under Metal (Cu, Zn and Fe) Toxicity in Arabidopsis thaliana

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Tarih

2020

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info:eu-repo/semantics/openAccess

Özet

Aim of this work was to investigate effects of toxic levels of Cu, Zn and Fe treatments on small ubiquitinlike modifier (SUMO) machinery of Arabidopsis thaliana. SUMO is a 100-115 amino acid posttranslational modifier that can regulate stability, activity or sub-cellular localization of target proteins. A.thaliana plants were treated with 50 µM Cu, 700 µM Zn and 400 µM Fe for 7 d and then expressions ofgenes related to SUMOylation and deSUMOylation of target proteins were measured with qRT-PCR.Only Cu treatment was able to induce genes related to SUMOylation (SUM3, SAE2, SIZ1) of targetproteins, while all of the three metals used in this study was effective in inducing a deSUMOylationrelated gene. Results of this study indicate that deSUMOylation of proteins might be a part of plantresponse to metal toxicity.

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Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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Cilt

16

Sayı

3

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