Optimization of serotonin imprinted polymers and recognition study from platelet rich plasma

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dc.contributor.authorOkutucu B.
dc.contributor.authorTelefoncu A.
dc.date.accessioned2019-10-26T23:56:32Z
dc.date.available2019-10-26T23:56:32Z
dc.date.issued2008
dc.departmentEge Üniversitesien_US
dc.description.abstractMolecularly imprinted polymers using serotonin as the template molecule was prepared for selective recognition from platelet rich plasma by non-covalent imprinting approach. Four different monomers (methacrylic acid, acrylamide, 4-vinylpyridine and 2-acrylamido-2-methylpropane sulfonic acid) and acetonitrile and DMSO as porogen were investigated for the first time by bulk polymerization. The molecularly imprinted polymer which was prepared by acrylamide/methacrylic acid had the largest imprinting factor for serotonin. The affinity and specificity of these polymers were evaluated by equilibrium binding experiments. The effect of polarity of the solvents was examined by polymers binding capacity and imprinting factor. According to the Scatchard analysis the Kd and Qmax values were calculated as 1.95 µmol l-1 and 19.129 µmol g-1, respectively. The polymer was tested to evaluate serotonin from platelet rich plasma and 70% serotonin recovery was found. © 2008 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorship2003FEN024en_US
dc.description.sponsorshipThis work was supported by Ege University Research Foundation 2003FEN024. --en_US
dc.identifier.doi10.1016/j.talanta.2008.05.033en_US
dc.identifier.endpage1158en_US
dc.identifier.issn0039-9140
dc.identifier.issue5en_US
dc.identifier.pmid18761170en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1153en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2008.05.033
dc.identifier.urihttps://hdl.handle.net/11454/20824
dc.identifier.volume76en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMolecular imprintingen_US
dc.subjectMolecular recognitionen_US
dc.subjectRebinding characteristicsen_US
dc.subjectSerotoninen_US
dc.titleOptimization of serotonin imprinted polymers and recognition study from platelet rich plasmaen_US
dc.typeArticleen_US

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