Evaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine

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dc.authoridBedir, Erdal/0000-0003-1262-063X
dc.authoridYakubogullari, Nilgun/0000-0002-5621-0221
dc.authorwosidBedir, Erdal/ABI-3895-2020
dc.contributor.authorYakubogullari, Nilgun
dc.contributor.authorCoven, Furkan Ozan
dc.contributor.authorCebi, Nusin
dc.contributor.authorCoven, Fethiye
dc.contributor.authorCoven, Nejdet
dc.contributor.authorGenc, Rukan
dc.contributor.authorBedir, Erdal
dc.date.accessioned2023-01-12T19:54:28Z
dc.date.available2023-01-12T19:54:28Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractAstragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [1139B411402292]en_US
dc.description.sponsorshipThis study partially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under Grant 1139B411402292.en_US
dc.identifier.doi10.1016/j.biologicals.2021.01.005
dc.identifier.endpage37en_US
dc.identifier.issn1045-1056
dc.identifier.issn1095-8320
dc.identifier.pmid33608170en_US
dc.identifier.startpage28en_US
dc.identifier.urihttps://doi.org/10.1016/j.biologicals.2021.01.005
dc.identifier.urihttps://hdl.handle.net/11454/76432
dc.identifier.volume70en_US
dc.identifier.wosWOS:000642444000005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofBiologicalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVaccine adjuvanten_US
dc.subjectNatural productsen_US
dc.subjectTriterpenoid saponinen_US
dc.subjectAstragaloside VIIen_US
dc.subjectAstragalus polysaccharideen_US
dc.subjectNewcastle disease virusen_US
dc.subjectIn-Vitroen_US
dc.subjectTriterpene Glycosidesen_US
dc.subjectSaponinsen_US
dc.subjectPolysaccharidesen_US
dc.subjectRootsen_US
dc.subjectQs-21en_US
dc.titleEvaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccineen_US
dc.typeArticleen_US

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