Determination of nitric oxide synthase activity and apoptosis of germ cells in different obstruction models

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dc.contributor.authorOktem, Gulperi
dc.contributor.authorAltay, Baris
dc.contributor.authorTurna, Burak
dc.contributor.authorAktug, Huseyin
dc.contributor.authorYavasoglu, Altug
dc.contributor.authorYilmaz, Zlem
dc.contributor.authorSemerci, Buelent
dc.date.accessioned2019-10-27T20:19:42Z
dc.date.available2019-10-27T20:19:42Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractWe aimed to determine the changes of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) immunoreactivity and apoptosis after proximal and distal obstruction models on ipsilateral and contralateral testicular tissues. Mate albino Wistar rats were randomly divided into three groups (n = 30): a control group which underwent sham operations (n = 10), a unilateral vasal ligation (n = 10) and a unilateral epididymal ligation group (n = 10). iNOS and eNOS distribution and apoptosis were studied in both ipsilateral and contralateral testes using quantitative immunohistochemistry. Nitric oxide synthase activity was significantly affected in ipsilateral and contralateral testes cells after vasal and epididymal ligation. eNOS immunoreactivity increased markedly after ipsilateral vasal ligation (ILVL). Degeneration-related changes were also associated with changes in apoptotic rate. Analysis using the terminal dUTP nick end-labeling TUNEL method revealed that apoptotic cell numbers significantly increased after ILVL. p53 and bcl-2 immunoreactivity increased in both experimental groups compared with the sham-operated group. Changes in iNOS and eNOS immunolocalisation were strongly associated with cell damage, because germ cell degeneration was more prominent in the ILVL group. Altered p53 immunolocalisation was also associated with cell degeneration, and a rise in bcl-2 immunoreactivity might be considered to reflect a protective mechanism in the testis. These cellular changes could enlighten understanding of the interaction between testicular functioning and damage. (c) 2008 Elsevier GmbH. All. rights reserved.en_US
dc.identifier.doi10.1016/j.acthis.2007.01.005en_US
dc.identifier.endpage126en_US
dc.identifier.issn0065-1281
dc.identifier.issue2en_US
dc.identifier.pmid18614207en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage119en_US
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2007.01.005
dc.identifier.urihttps://hdl.handle.net/11454/41486
dc.identifier.volume111en_US
dc.identifier.wosWOS:000265148700004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbh, Urban & Fischer Verlagen_US
dc.relation.ispartofActa Histochemicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTestisen_US
dc.subjectEpididymal ligationen_US
dc.subjectVasal ligationen_US
dc.subjectINOSen_US
dc.subjectENOSen_US
dc.subjectImmunohistochemistryen_US
dc.subjectApoptosisen_US
dc.titleDetermination of nitric oxide synthase activity and apoptosis of germ cells in different obstruction modelsen_US
dc.typeArticleen_US

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