Arabidopsis NATA1 Acetylates Putrescine and Decreases Defense-Related Hydrogen Peroxide Accumulation

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dc.contributor.authorLou, Yann-Ru
dc.contributor.authorBor, Melike
dc.contributor.authorYan, Jian
dc.contributor.authorPreuss, Aileen S.
dc.contributor.authorJander, Georg
dc.date.accessioned2019-10-27T23:09:32Z
dc.date.available2019-10-27T23:09:32Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractBiosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions in mammalian cells, has been observed in several plant species. Here we show that Arabidopsis (Arabidopsis thaliana) N-ACETYLTRANSFERASE ACTIVITY1 (NATA1) catalyzes acetylation of putrescine to N-acetylputrescine and thereby competes with spermidine synthase for a common substrate. NATA1 expression is strongly induced by the plant defense signaling molecule jasmonic acid and coronatine, an effector molecule produced by DC3000, a Pseudomonas syringae strain that initiates a virulent infection in Arabidopsis ecotype Columbia-0. DC3000 growth is reduced in nata1 mutant Arabidopsis, suggesting a role for NATA1-mediated putrescine acetylation in suppressing antimicrobial defenses. During infection by P. syringae and other plant pathogens, polyamine oxidases use spermidine and spermine as substrates for the production of defense-related H2O2. Compared to wild-type Columbia-0 Arabidopsis, the response of nata1mutants to P. syringae infection includes reduced accumulation of acetylputrescine, greater abundance of nonacetylated polyamines, elevated H2O2 production by polyamine oxidases, and higher expression of genes related to pathogen defense. Together, these results are consistent with a model whereby P. syringae growth is improved in a targeted manner through coronatine-induced putrescine acetylation by NATA1.en_US
dc.description.sponsorshipUS National Science FoundationNational Science Foundation (NSF) [1121788, 1022017]; Fulbright fellowship; Guangzhou City [2013J2200082]; Deutscher Akademischer Austauschdienst-Research Internships in Science and Engineering (DAAD-RISE) fellowship; Science and Technology Star of Zhujiangen_US
dc.description.sponsorshipThis research was funded by US National Science Foundation awards 1121788 and 1022017 to G.J., a Fulbright fellowship to M.B., a fellowship from Science and Technology Star of Zhujiang, Guangzhou City (#2013J2200082) to J.Y., and a Deutscher Akademischer Austauschdienst-Research Internships in Science and Engineering (DAAD-RISE) fellowship to A.S.P.en_US
dc.identifier.doi10.1104/pp.16.00446en_US
dc.identifier.endpage1455en_US
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.issue2en_US
dc.identifier.pmid27208290en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1443en_US
dc.identifier.urihttps://doi.org/10.1104/pp.16.00446
dc.identifier.urihttps://hdl.handle.net/11454/52674
dc.identifier.volume171en_US
dc.identifier.wosWOS:000380699200052en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Plant Biologistsen_US
dc.relation.ispartofPlant Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleArabidopsis NATA1 Acetylates Putrescine and Decreases Defense-Related Hydrogen Peroxide Accumulationen_US
dc.typeArticleen_US

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