Cytotoxicity of Silica Nanoparticles with Transcaucasian Nose-Horned Viper, Vipera ammodytes transcaucasiana, Venom on U87MG and SHSY5Y Neuronal Cancer Cells

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dc.contributor.authorCelen, Cigdem
dc.contributor.authorKececiler, Ceren
dc.contributor.authorKaris, Mert
dc.contributor.authorGocmen, Bayram
dc.contributor.authorYesil-Celiktas, Ozlem
dc.contributor.authorNalbantsoy, Ayse
dc.date.accessioned2019-10-27T10:02:10Z
dc.date.available2019-10-27T10:02:10Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractHighly bioactive compounds of the snake venom make them particular sources for anticancer agent development. They contain very rich peptide-protein structures. Therefore, they are very susceptible to environmental conditions such as temperature, pH, and light. In this study, Vipera ammodytes transcaucasiana venom was encapsulated in PAMAM-G4 dendrimer by sol-gel method in order to prevent degradation of venom contents from the environmental conditions. For this purpose, nanoparticles were prepared by sol-gel methodology and SEM analyses were performed. U87MG and SHSY5Y neuronal cancer cell lines were treated with different concentrations of venom-containing nanoparticles and cytotoxicity was determined by MTT assay. IC50 values of nanoparticles with snake venom were calculated as 37.24 and 44.64g/ml for U87MG and SHSY5Y cells, respectively. The IC50 values of nanoparticles with snake venom were calculated as 10.07 and 7.9g/ml for U87MG and SHSY5Y cells, respectively. As a result, nanoparticles with V. a. transcaucasiana venom showed remarkably high cytotoxicity. Encapsulation efficiency of nanoparticles with 1mg/ml snake venom was determined as %67 via BCA protein analysis. In conclusion, this method is found to be convenient and useful for encapsulating snake venom as well as being suitable for drug delivery systems.en_US
dc.identifier.doi10.1007/s12010-018-2742-2en_US
dc.identifier.endpage357en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue2en_US
dc.identifier.pmid29611136en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage350en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-018-2742-2
dc.identifier.urihttps://hdl.handle.net/11454/29955
dc.identifier.volume186en_US
dc.identifier.wosWOS:000443820700005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSnake venomen_US
dc.subjectEncapsulationen_US
dc.subjectSol-gelen_US
dc.subjectCytotoxicityen_US
dc.subjectNerve cellsen_US
dc.titleCytotoxicity of Silica Nanoparticles with Transcaucasian Nose-Horned Viper, Vipera ammodytes transcaucasiana, Venom on U87MG and SHSY5Y Neuronal Cancer Cellsen_US
dc.typeArticleen_US

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