Increased alkalotolerant and thermostable ribonuclease (RNase) production from alkaliphilic Streptomyces sp. M49-1 by optimizing the growth conditions using response surface methodology

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dc.contributor.authorDemir T.
dc.contributor.authorGübe Ö.
dc.contributor.authorYücel M.
dc.contributor.authorHameş-Kocabaş E.E.
dc.date.accessioned2019-10-27T08:23:18Z
dc.date.available2019-10-27T08:23:18Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractTotal of 171 alkaliphilic actinomycetes were evaluated for extracellular RNase production and Streptomyces sp. M49-1 was selected for further experiments. Fermentation optimization for RNase production was implemented in two steps using response surface methodology with central composite design. In the first step, the effect of independent fermentation variables including temperature, initial pH and process time were investigated. After identification of carbon and nitrogen sources affecting the production by one variable at a time method, concentrations of glucose and yeast extract and also inoculum size were chosen for the second central composite design. A maximum RNase activity was obtained under optimal conditions of 4.14 % glucose concentration, 4.63 % yeast extract concentration, 6.7 × 106 spores as inoculum size for 50 ml medium, 42.9 °C, 91.2 h process time and medium initial pH 9.0. Optimum activity of the enzyme is achieved at pH 11 and temperature 60 °C. The enzyme is highly stable at pH range 9.0-12.0 and at 90 °C after 2 h. Statistical optimization experiments provide 2.25 fold increases in the activity of alkalotolerant and thermostable RNase and shortened the fermentation time compared to that of unoptimized condition. The members of Streptomyces can be promising qualified RNase producer for pharmaceutical industries. © 2013 Springer Science+Business Media Dordrecht.en_US
dc.description.sponsorshipAcknowledgments This research was supported by The Research Fund of Ege University with 09.MUH.078 project number. The authors would like to thank Professor Murat Elibol (Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey) for his valuable comments and suggestions during the preparation of this manuscript. --en_US
dc.identifier.doi10.1007/s11274-013-1325-1en_US
dc.identifier.endpage1633en_US
dc.identifier.issn0959-3993
dc.identifier.issue9en_US
dc.identifier.pmid23532461en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1625en_US
dc.identifier.urihttps://doi.org/10.1007/s11274-013-1325-1
dc.identifier.urihttps://hdl.handle.net/11454/26405
dc.identifier.volume29en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofWorld Journal of Microbiology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlkalotolerant and thermostable RNaseen_US
dc.subjectCentral composite designen_US
dc.subjectOptimizationen_US
dc.subjectResponse surface methodologyen_US
dc.subjectStreptomycesen_US
dc.titleIncreased alkalotolerant and thermostable ribonuclease (RNase) production from alkaliphilic Streptomyces sp. M49-1 by optimizing the growth conditions using response surface methodologyen_US
dc.typeArticleen_US

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