Effect of gamma-glutamylcysteine ethylester on the levels of c-fos mRNA expression, glutathione and reactive oxygen species formation in kainic acid excitotoxicity

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dc.contributor.authorTurunc, Ezgi
dc.contributor.authorKanit, Lutfiye
dc.contributor.authorYalcin, Ayfer
dc.date.accessioned2019-10-27T21:16:06Z
dc.date.available2019-10-27T21:16:06Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives The aim of this study was to investigate the effect of gamma-glutamylcysteine ethylester (GCEE), a precursor of glutathione biosynthesis, on the levels of glutathione, formation of reactive oxygen species and c-fos mRNA expression in rat hippocampus and cortex in kainic acid-induced excitotoxicity. Methods Sprague-Dawley rats were used and divided into four groups: control, kainic acid (10 mg/kg), GCEE (10 mg/kg) and kainic acid (10 mg/kg) + GCEE (10 mg/kg). Kainic acid and GCEE were administered to the rats intraperitoneally. The levels of glutathione and the expressions of c-fos mRNA in hippocampus and cortex tissues were determined using spectrophotometric and reverse transcription followed real-time PCR methods, respectively. Formation of reactive oxygen species was determined using dichlorofluorescin fluorescence in brain synaptosomes treated with kainic acid or GCEE in vitro. Key findings Kainic acid treatment significiantly upregulated the expression of c-fos mRNA in the hippocampus and cortex when compared to the control group. GCEE treatment significantly decreased the levels of c-fos mRNA in the cortex when compared to the kainic acid-treated group. GCEE treatment against kainic acid significantly increased the levels of glutathione in the cortex and hippocampus, and decreased the levels of formation of reactive oxygen species when compared to kainic acid-treated synaptosomes. Conclusions The increased levels of glutathione and the reduced levels of reactive oxygen species formation lead us to conclude that GCEE may be beneficial as a potential antioxidant against neurodegenerative processes where excitotoxicity is involved.en_US
dc.description.sponsorshipTechnological and Scientific Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-104S280]; Ege University Research FoundationEge University [2005/ECZ 009]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis work was supported by the Technological and Scientific Council of Turkey (TUBITAK) (SBAG-104S280 to A. Y.) and the Ege University Research Foundation (2005/ECZ 009 to A. Y.). E. T. was also supported by doctoral grant from TUBITAK.en_US
dc.identifier.doi10.1111/j.2042-7158.2010.01122.xen_US
dc.identifier.endpage1017en_US
dc.identifier.issn0022-3573
dc.identifier.issn2042-7158
dc.identifier.issue8en_US
dc.identifier.pmid20663035en_US
dc.identifier.startpage1010en_US
dc.identifier.urihttps://doi.org/10.1111/j.2042-7158.2010.01122.x
dc.identifier.urihttps://hdl.handle.net/11454/43623
dc.identifier.volume62en_US
dc.identifier.wosWOS:000280202300007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Pharmacy and Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgamma-glutamylcysteine ethylesteren_US
dc.subjectc-fos mRNAen_US
dc.subjectglutathioneen_US
dc.subjectkainic aciden_US
dc.subjectrat brainen_US
dc.subjectreactive oxygen speciesen_US
dc.titleEffect of gamma-glutamylcysteine ethylester on the levels of c-fos mRNA expression, glutathione and reactive oxygen species formation in kainic acid excitotoxicityen_US
dc.typeArticleen_US

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