Evaluation of cell protection by Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage

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dc.authoridDagci, Taner/0000-0002-2786-6141
dc.authorwosidDagci, Taner/A-9827-2008
dc.authorwosidArmagan, Guliz/ABI-5508-2020
dc.contributor.authorTastan, Pelin
dc.contributor.authorArmagan, Guliz
dc.contributor.authorDagci, Taner
dc.contributor.authorKivcak, Bijen
dc.date.accessioned2023-01-12T19:59:05Z
dc.date.available2023-01-12T19:59:05Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractNeurodegenerative diseases affect millions of people. Major reasons behind the onset and progression of these diseases are still under investigation. Therefore, any approach that would treat/prevent progression is important. In this study, we aimed to investigate the potential protective effects of Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damage and compare the effectiveness of different extracts (methanol:water (1:1), chloroform and n-hexane). The cells were pretreated with four different concentrations (10, 50, 100, and 200 mu g/ml) of methanol:water (1:1), chloroform and n-hexane extracts of P. pyrrhoblepharus following MPP+ treatment for 12 or 24 h. The changes in cell viability were determined using the MTT assay. Additionally, antioxidant activities and total phenolic/flavonoid contents of the extracts were determined with radical scavenging capacity, Folin-Ciocalteu and aluminum chloride assays, respectively. The extracts at selected concentrations were found to be protective in a dose-dependent manner at 12 and 24 h. Nevertheless, the methanol extract of the plant showed the highest protection both at 100 and 200 mu g/ml (115.13%+/- 3.98, 121.87%+/- 1.66; p < 0.05) against dopaminergic damage at 24 h. The results showed that selected concentrations were not toxic and did not affect cell proliferation rate. Besides, the chloroform extract was found to have higher antioxidant activity than the other extracts (p < 0.05). The total phenolic and total flavonoid contents were found consistent with antioxidant activities. Our findings support the neuroprotective and antioxidant potential of P. pyrrhoblepharus. However, further studies on identifying the presence of chemicals in P. pyrrhoblepharus extracts which are responsible for protection should be carried out to confirm their therapeutic potential.en_US
dc.identifier.doi10.1080/01480545.2019.1659808
dc.identifier.endpage76en_US
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue1en_US
dc.identifier.pmid31474160en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://doi.org/10.1080/01480545.2019.1659808
dc.identifier.urihttps://hdl.handle.net/11454/77093
dc.identifier.volume45en_US
dc.identifier.wosWOS:000484501300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofDrug and Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPsephellus pyrrhoblepharusen_US
dc.subjectantioxidant activityen_US
dc.subjectMPP+en_US
dc.subjectdopaminergic cell deathen_US
dc.subjectneuroprotectionen_US
dc.subjectSesquiterpene-Lactonesen_US
dc.subjectSubtribe Centaureinaeen_US
dc.subjectPhenolic-Compoundsen_US
dc.subjectAntioxidanten_US
dc.subjectNeurotoxicityen_US
dc.subjectFlavonoidsen_US
dc.subjectApoptosisen_US
dc.subjectModelen_US
dc.subjectAciden_US
dc.titleEvaluation of cell protection by Psephellus pyrrhoblepharus (Boiss.) Wagenitz extracts in MPP+-induced dopaminergic cell damageen_US
dc.typeArticleen_US

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