Testing of nonlinear electrofrictiophoresis in agarose gel

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dc.contributor.authorVidybida, AK
dc.contributor.authorErikzan, V
dc.contributor.authorAltungoz, O
dc.contributor.authorCelebi, G
dc.date.accessioned2019-10-27T18:22:22Z
dc.date.available2019-10-27T18:22:22Z
dc.date.issued2000
dc.departmentEge Üniversitesien_US
dc.description.abstractIt was theoretically predicted earlier that if a periodic force without constant component is applied to a particle, then the particle can produce a directed drift in some direction. The effect is named nonlinear electrofrictiophoresis, because it is crucial for its appearance that the friction force depends on the particle's velocity in a nonlinear manner. We test a possibility to observe this effect when a mixture of fragments of DNA (the DNA ladder) moves in the agarose gel. For this purpose, we study the nonlinear characteristics of a DNA ladder movement in the gel. The gels with the ladder were run under various electric field strengths. It was found that the friction coefficient for each DNA fragment in the ladder depends on the migration velocity, suggesting that energy dissipation during migration is a nonlinear function of velocity. This nonlinearity makes the system under consideration suitable for observing nonlinear electrofrictiophoresis, A possible velocity of directed drift under periodic electric drive without constant component was estimated numerically for experimentally observed dependencies. The velocity appeared to be comparable with that of migration under a constant field of moderate strength. A possible mechanism of energy dissipation during movement of DNA through the gel is discussed. (C) 2000 Elsevier Science S.A. All rights reserved.en_US
dc.identifier.doi10.1016/S0302-4598(00)00088-Xen_US
dc.identifier.endpage101en_US
dc.identifier.issn0302-4598
dc.identifier.issue1en_US
dc.identifier.pmid11059582en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://doi.org/10.1016/S0302-4598(00)00088-X
dc.identifier.urihttps://hdl.handle.net/11454/35996
dc.identifier.volume52en_US
dc.identifier.wosWOS:000167099600013en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectnonlinear frictionen_US
dc.subjectelectrofrictiophoresisen_US
dc.subjectDNAen_US
dc.subjectagarose gelen_US
dc.titleTesting of nonlinear electrofrictiophoresis in agarose gelen_US
dc.typeArticleen_US

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