The effect of phytoestrogens on fracture healing: experimental research in new zealand white rabbits
Küçük Resim Yok
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Fitoöstrojenler bitkisel kaynakl› do¤al moleküller olup kemik yap›m›n› art›r›c› ve kemik yerine kullan›labilme özellikleri vard›r. Bu çal›flmada, fitoöstrojenlerin tavflan k›r›k modelinde k›r›k iyileflmesi üzerine etkileri deneysel olarak araflt›r›ld›. GEREÇ VE YÖNTEM Yirmi iki adet Yeni Zelanda beyaz tavflan› sa¤ tibia k›r›¤› oluflturulduktan sonra randomize olarak iki gruba ayr›ld›. Birinci gruba Vitex agnus-castus L.’den (Verbenaceae) çal›flma öncesinde haz›rlanan bitki ekstresi intramuskuler olarak enjekte edildi. ‹kinci grup kontrol grubu olarak seçildi. K›r›k iyileflmesi haftal›k olarak kan alkalen fosfataz düzeyi, direkt radyografi ve 99m-Tc MDP kemik sintigrafisi ile de¤erlendirildi. Üçüncü haftan›n sonunda çal›flma sonland›r›ld›. Çal›flma sonunda histopatolojik inceleme yap›ld›. BULGULAR Yedinci günde yap›lan direkt radyografik incelemede k›r›k iyileflme bulgular› grup 1’de kontrol grubuna göre daha yüksek olarak bulundu (p<0,01). Yedinci günde yap›lan 99m-Tc MDP kemik sintigrafisinde grup 1’de tutulum oranlar› kontrol grubuna göre daha fazla idi (p<0,05). Grup 1’de 14. ve 7. gün 99m- Tc MDP tutulum miktarlar› aras›ndaki fark kontrol grubuna göre daha fazla bulundu (p=0,04). Yirmi beflinci günde yap›lan histopatolojik incelemede gruplar aras›nda anlaml› farkl›l›k yoktu. Di¤er de¤iflkenler aras›nda farkl›l›k saptanmad›. SONUÇ Flavonoid enjeksiyonlar›n›n Yeni Zelanda beyaz tavflanlar›nda k›r›k iyileflmesinin erken döneminde faydal› oldu¤u gösterildi. Farkl› doz uygulamalar› ve verilifl yöntemlerinin farkl› deneysel çal›flmalarla araflt›r›lmas›n› önermekteyiz.
Phytoestrogens are plant-derived natural molecules having some bone forming and bone substituting effects. In the present study, the role of phytoestrogens on bone healing was investigated in a rabbit fracture model. METHODS Twenty-two New Zealand white rabbits with right tibia fracture were divided into two groups randomly. The plant derived extract of Vitex agnus-castus L. (Verbenaceae) prepared before the study was administered intramuscularly in group 1 and group 2 was chosen as control. Fracture healing was monitored in weekly basis with blood alkaline phosphatase level, radiographs of extremities and 99m-Tc MDP bone scintigraphy. The study was finished at the end of the 3rd week. The extremities including tibial fractures were collected for histological examination. RESULTS Radiographic evidence of fracture healing obtained on postoperative day seven was superior in group 1 than control group (p<0.01). The 99m-Tc MDP bone scintigraphy uptake ratios on postoperative seventh day showed higher uptake in group 1 than in group 2 (p<0.05). The differences of scintigraphic uptakes in fractured tibias calculated on postoperative seventh day and postoperative 14th in group 1 were higher than group 2 (p=0.04). The histopathologic evaluation performed after sacrification of all rabbits on postoperative 25th day showed no significant difference between both groups. No statistical diff e r e nce was determined related to the other variables. CONCLUSION Flavonoids affected positively the early periods of fracture healing mechanism in New Zealand white rabbits. We suggest further stud ies with phytoe st r ogens to det e rm ine the effects of var i ous dosages and administration ways.
Phytoestrogens are plant-derived natural molecules having some bone forming and bone substituting effects. In the present study, the role of phytoestrogens on bone healing was investigated in a rabbit fracture model. METHODS Twenty-two New Zealand white rabbits with right tibia fracture were divided into two groups randomly. The plant derived extract of Vitex agnus-castus L. (Verbenaceae) prepared before the study was administered intramuscularly in group 1 and group 2 was chosen as control. Fracture healing was monitored in weekly basis with blood alkaline phosphatase level, radiographs of extremities and 99m-Tc MDP bone scintigraphy. The study was finished at the end of the 3rd week. The extremities including tibial fractures were collected for histological examination. RESULTS Radiographic evidence of fracture healing obtained on postoperative day seven was superior in group 1 than control group (p<0.01). The 99m-Tc MDP bone scintigraphy uptake ratios on postoperative seventh day showed higher uptake in group 1 than in group 2 (p<0.05). The differences of scintigraphic uptakes in fractured tibias calculated on postoperative seventh day and postoperative 14th in group 1 were higher than group 2 (p=0.04). The histopathologic evaluation performed after sacrification of all rabbits on postoperative 25th day showed no significant difference between both groups. No statistical diff e r e nce was determined related to the other variables. CONCLUSION Flavonoids affected positively the early periods of fracture healing mechanism in New Zealand white rabbits. We suggest further stud ies with phytoe st r ogens to det e rm ine the effects of var i ous dosages and administration ways.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Ulusal Travma ve Acil Cerrahi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
14
Sayı
1
