The influence of marginal zinc deficient diet on post-vaccination immune response against hepatitis B in rats

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dc.contributor.authorOzgenc, Funda
dc.contributor.authorAksu, Guzide
dc.contributor.authorKirkpinar, Figen
dc.contributor.authorAltuglu, Imre
dc.contributor.authorCoker, Isil
dc.contributor.authorKutukculer, Necil
dc.contributor.authorYagci, Rasit Vural
dc.date.accessioned2019-10-27T19:18:46Z
dc.date.available2019-10-27T19:18:46Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: To evaluate in vitro T lymphocyte proliferation and specific antibody response to hepatitis B vaccination in two groups of rats fed with normal and marginal zinc content. Methods: Twenty-two Wistar-Albino rats were randomly assigned into two groups and were fed with constant diet. Zinc was suplemented 10 mg/kg dry weight in group I (marginal zinc content) (n = 14) and 30 mg/kg dry weight in group II (n = 8). Hepatitis B vaccine (Engerix B, 4 mu g) was administered intramuscularly after 8 weeks on feeding and a booster dose was applied 4 weeks after the first injection. Rats were killed 3 weeks after the second injection. Peripheral blood mononuclear cells were stimulated in vitro by PHA (2.5 mu g/ml) and hepatitis B surface antigen (2.5, 5, 10 mu g/ml). Proliferation was evaluated by ELISA (celltiter-96 aqueous one solution cell proliferation assay). Serum zinc, anti-HBs titer and zinc per dry liver weight were also measured. Two groups were compared with respect to antigen specific antibody and lymphocyte proliferation responses. Proliferation response to HbsAg were expressed as net percent increase (pci) in lymphocyte proliferation from the baseline activity. Results: Rats' mean body weight and weight gain per month were similar. Median serum zinc was 39 (23-75) and 76 (64-115) mu g/dl of groups I and 11 rats, respectively (p < 0.05), while there was no difference in liver zinc content between the two groups (37 mu g/g dry weight versus 32 mu g/g dry weight). Median anti-HBs levels of groups I and II were 741 (0-10,000) IU/l, 5791 (558-10,000) IU/l, respectively (P < 0.05). In lymphocyte proliferation assays, mean net pci with HbsAg of 5 and 10 mu g/ml were 9.4% and 11.3% in group I rats; while they were 25.3% and 26.1% in group 11 rats (p < 0.01 and p < 0.01, respectively). Conclusion: In vitro cell-mediated immune response and in vivo specific antibody response to hepatitis B vaccine was decreased in rats fed a diet with marginal zinc content. These observations have shown that marginal Zn deficiency might influence the efficacy of hepatitis B vaccination in humans. (c) 2006 Elsevier Ireland Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.hepres.2006.01.012en_US
dc.identifier.endpage30en_US
dc.identifier.issn1386-6346
dc.identifier.issn1872-034X
dc.identifier.issue1en_US
dc.identifier.pmid16600672en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.1016/j.hepres.2006.01.012
dc.identifier.urihttps://hdl.handle.net/11454/38641
dc.identifier.volume35en_US
dc.identifier.wosWOS:000237619600005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofHepatology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthepatitis Ben_US
dc.subjectzincen_US
dc.subjectvaccinationen_US
dc.subjectimmune responseen_US
dc.subjectin vitro lymphocyte proliferationen_US
dc.titleThe influence of marginal zinc deficient diet on post-vaccination immune response against hepatitis B in ratsen_US
dc.typeArticleen_US

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