Futbolcularda ve sedanter bireylerde telomeraz aktivitesi ile ACE ID gen polimorfizmi arasındaki ilişki
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ege Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
ÖZET Araştırmanın Adı: Futbolcularda ve Sedanter Bireylerde Telomeraz Aktivitesi İle ACE ID Gen Polimorfizmi Arasındaki İlişki. Amaç: Futbolcularda ve sedanter bireylerde ACE ID gen polimorfizmi ile Maks VO2arasındaki ilişkiyi belirlemektir. Yöntem:Çalışmaya 18-30 yaş arası, benzer karakteristik özelliklere sahip 104 sağlıklı erkek futbolcu ve kontrol grubu için 103 sağlıklı sedanter erkek birey alındı. Kişilerin tıbbi öyküleri alındı ve fizik muayeneleri yapıldı. Tüm olgulara skinfold kaliperle bölgesel yağ ölçümü, biyoempedans ile vücut kompozisyonu ölçümleri yapıldı. Treadmille bağlı Type Masterscreen CPX aleti ile Maks VO2 ölçümü yapıldı.ACE ID gen polimorfizmiiçin EDTA'lı tübe kan alındı.Polimeraz Zincir Reaksiyonu (PCR) metodu kullanılarak ACE ID gen polimorfizmi analizi yapıldı.İstatistiksel değerlendirmede tanımlayıcı analizler dışında hem sporun etkisini hem de gen polimorfizminin etkisini görmek için 2x3 Faktöriyel ANOVA analizi yapılmıştır. Gen dağılımı için Hardy-Weinberg analizi yapılmıştır. İstatistiksel anlamlılık sınırı p<0,05 olarak kabul edilmiştir. Bulgular: Vücut kompozisyonu için Vücut Kitle İndeksi (VKİ), Vücut Yağ Oranı (VYO) ve Skinfold ile yapılan ölçümlerin tümünde futbolcu grubunun ortalama değerleri kontrol grubununkilerden daha düşük saptanmıştır. Kontrol grubunun Total (ml/dk) ve kilogram başına (ml/kg/dk) Solunumsal Eşik ile total (ml/dk) ve kilogram başına (ml/kg/dk) Maks VO2değerleri futbolcu grubuna göre anlamlı derecede düşük saptanmıştır. Tüm kişilerde ACE ID gen polimorfizmi dağılımına bakıldığında, ACE II gen polimorfizmi için % 21,7, ACE ID gen polimorfizmi için % 45,5, ACE DD gen polimorfizmi için % 32,8 dir. Allel dağılımı ise % 44,4 I alleli, % 55,6 D alleli olarak saptanmış olup, futbolu grubu ile kontrol grubu arasında gen dağılımı ve allel oranı açısından fark saptanmamıştır.ACE ID gen polimorfizmi ile Maks VO2 arasında ilişki saptanmamıştır. Bununla birlikte tüm bireylerde ACE II gen polimorfizmine sahip bireylerin Maks VO2 değeri ortalaması (52,1 ± 10,3 ml/kg/dk), ACE ID (50,5 ± 9,6 ml/kg/dk) ve DD'ye (50,9 ± 10,8 ml/kg/dk) sahip bireylere göre yüksek saptanmıştır. Ancak bu fark istatistiksel olarak anlamlı değildir.Bu tez çalışmasında ACE ID gen polimorfizminin vücut kompozisyonu üzerine etkisinin gözlendiği tek yer subskapula bölgesindeki skinfold değeri olmuştur. ACE ID gen polimorfizminin, vücut ağırlığı (VA), VKİ, VYO ve skinfoldun diğer bölgelerinin ölçümü üzerinde etkisi saptanmamıştır.Maks VO2 (özellikle kilogram başına) değerleri ile vücut kompozisyonu ölçümleri (VA, VKİ, VYO, skinfold değerleri) arasında negatif korelasyon saptanmıştır. Sonuç: Bu tez çalışmasında, futbolcularda ve sedanter bireylerde ACE ID gen polimorfizmi ile vücut kompozisyonu ölçümlerinde ve Maks VO2 arasında ilişki saptanmamıştır
SUMMARY Research Title:Relationship between Telomerase Activity and ACE ID gene Polymorphism in soccer players and sedentary individuals. Purpose:The main objective of this research is to determine the relationship between VO2 Max. and ACE ID gene polymorphism in football players and sedentary subjects. Methods: 104 healty football players and 103 healty sedentary induviduals who are at ages between 18-30 and have similar characteristics were joined to the study. Participant's medical histories were taken and physical examinations were performed. Regional fat measurement by skinfold caliper and body composition measurements by bioimpedance were made to all induviduals who joined to the study. VO2 Max measurements were performed with Type Masterscreen CPX that connected to a treadmille. Blood was taken into EDTA tubes for ACE ID gene polymorphism. ACE ID gene polymorphism analysis was performed using the PCR method. Apart from descriptive analysis, 2x3 factorial ANOVA analysis was performed to see the effect of both sports and gene polymorphism in statistical evaluation. Hardy-Weinberg analysis was made for gene distribution. Statistical significance p<0.05 were used. Results: To body composition, the football players' Body Mass Index (BMI), Body Fat Percentage (BFP) and all skinfold measurements average results is showed lower than the control group. Total (ml / min) and per kilogram (mL / kg / min) values of the Ventilatory Threshold and VO2 Max in control group was found significantly lower than football players. ACE ID gene polymorphism distribution in all people are 21.7% for ACE II, 45.5% for ACE ID, 32.8% for ACE DD. I and D alleles were found as 44.4% and 55.6%. Football players group and the control group weresimilar about the gene distribution. ACE ID gene polymorphism and VO2 Max is not associated with each other. However, average VO2 Max(52.1 ± 10.3 ml/kg/min) values of all individuals who has ACE II polymorphism was found higher than the ACE ID (50.5 ± 9.6 ml/kg/min) and DD (50.9 ± 10.8 ml/kg/min), but this difference is not statistically significant. In our study, the only body part that the effect of ACE ID gene polymorphism is observed on body composition is skinfold value at subscapula. Effect of ACE ID gene polymorphism on body weight, BMI, BFP and other skinfold measurements has not been determined. In VO2 Max (especially per kilogram) values and body composition measurements (body weight, BMI, BFP, skinfold values) negative correlation was found. Conclusion:According to our investigation, in football players and sedentary subjects, ACE ID gene polymorphism is not associated with VO2 Max and body composition measurements.
SUMMARY Research Title:Relationship between Telomerase Activity and ACE ID gene Polymorphism in soccer players and sedentary individuals. Purpose:The main objective of this research is to determine the relationship between VO2 Max. and ACE ID gene polymorphism in football players and sedentary subjects. Methods: 104 healty football players and 103 healty sedentary induviduals who are at ages between 18-30 and have similar characteristics were joined to the study. Participant's medical histories were taken and physical examinations were performed. Regional fat measurement by skinfold caliper and body composition measurements by bioimpedance were made to all induviduals who joined to the study. VO2 Max measurements were performed with Type Masterscreen CPX that connected to a treadmille. Blood was taken into EDTA tubes for ACE ID gene polymorphism. ACE ID gene polymorphism analysis was performed using the PCR method. Apart from descriptive analysis, 2x3 factorial ANOVA analysis was performed to see the effect of both sports and gene polymorphism in statistical evaluation. Hardy-Weinberg analysis was made for gene distribution. Statistical significance p<0.05 were used. Results: To body composition, the football players' Body Mass Index (BMI), Body Fat Percentage (BFP) and all skinfold measurements average results is showed lower than the control group. Total (ml / min) and per kilogram (mL / kg / min) values of the Ventilatory Threshold and VO2 Max in control group was found significantly lower than football players. ACE ID gene polymorphism distribution in all people are 21.7% for ACE II, 45.5% for ACE ID, 32.8% for ACE DD. I and D alleles were found as 44.4% and 55.6%. Football players group and the control group weresimilar about the gene distribution. ACE ID gene polymorphism and VO2 Max is not associated with each other. However, average VO2 Max(52.1 ± 10.3 ml/kg/min) values of all individuals who has ACE II polymorphism was found higher than the ACE ID (50.5 ± 9.6 ml/kg/min) and DD (50.9 ± 10.8 ml/kg/min), but this difference is not statistically significant. In our study, the only body part that the effect of ACE ID gene polymorphism is observed on body composition is skinfold value at subscapula. Effect of ACE ID gene polymorphism on body weight, BMI, BFP and other skinfold measurements has not been determined. In VO2 Max (especially per kilogram) values and body composition measurements (body weight, BMI, BFP, skinfold values) negative correlation was found. Conclusion:According to our investigation, in football players and sedentary subjects, ACE ID gene polymorphism is not associated with VO2 Max and body composition measurements.
Açıklama
Anahtar Kelimeler
Spor, Sports, Anjiyotensin konverting enzim, Angiotensin converting enzyme, Egzersiz, Exercise, Futbol, Football, Futbolcular, Football players, Oksijen, Oxygen, Oksijen tüketimi, Oxygen consumption, Polimorfizm-genetik, Polymorphism-genetic, Solunum, Respiration, Telomer, Telomere
