Enhancement of vinorelbine-induced cytotoxicity and apoptosis by clomipramine and lithium chloride in human neuroblastoma cancer cell line SH-SY5Y

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dc.contributor.authorBilir, Ayhan
dc.contributor.authorErguven, Mine
dc.contributor.authorYazihan, Nuray
dc.contributor.authorAktas, Esin
dc.contributor.authorOktem, Gulperi
dc.contributor.authorSabanci, Akin
dc.date.accessioned2019-10-27T21:29:21Z
dc.date.available2019-10-27T21:29:21Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this work is to investigate whether clomipramine (CIM) and lithium chloride (LiCl) potentiate the cytotoxicity of vinorelbine (VNR) on SH-SY5Y human neuroblastoma cells in vitro and whether midkine (MK) can be a resistance factor for these treatments. Four groups of experiments were performed for 96 h using both monolayer and spheroid cultures of SH-SY5Y cells: (1) control group, (2) singly applied VNR, CIM, and LiCl, (3) VNR with CIM, and (4) VNR with LiCl. Their effects on monolayer and spheroid cultures were determined by evaluating cell proliferation, bromodeoxyuridine labeling index (BrdU-LI), apoptosis, cyclic adenosine monophosphate (cAMP) and midkine levels, colony-forming efficiency, spheroid size, and ultrastructure. In comparison with the control group, single and combination drug treatments significantly reduced the proliferation index (PI) for 96 h. The most potent reduction of PI was observed with VNR in combination with CIM and LiCl for all time intervals. VNR with CIM and LiCl seemed to be ineffective in reducing BrdU-LI of both monolayer cell and spheroid cultures, spheroid size, and cAMP level. VNR with LiCl increased apoptosis at 24 h, however VNR with CIM increased apoptosis at 96 h. VNR was the most potent drug in inhibiting colony-forming efficiency. The combination of VNR with CIM was the most potent in reducing midkine levels among all groups. Interestingly, the combination of VNR with LiCl led to both nuclear membrane breakdown and disappearance of the cellular membranes inside the spheroids. Both CIM and LiCl seemed to potentiate VNR-induced cytotoxicity, and MK was not a resistance factor for VNR, LiCl, and CIM.en_US
dc.description.sponsorshipIstanbul UniversityIstanbul University [2877]en_US
dc.description.sponsorshipThis work was supported by Scientific Research Projects Coordination Unit of Istanbul University. Project number: 2877.en_US
dc.identifier.doi10.1007/s11060-010-0209-6en_US
dc.identifier.endpage395en_US
dc.identifier.issn0167-594X
dc.identifier.issue3en_US
dc.identifier.pmid20467784en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage385en_US
dc.identifier.urihttps://doi.org/10.1007/s11060-010-0209-6
dc.identifier.urihttps://hdl.handle.net/11454/45270
dc.identifier.volume100en_US
dc.identifier.wosWOS:000283370800006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Neuro-Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVinorelbineen_US
dc.subjectClomipramineen_US
dc.subjectLithium chlorideen_US
dc.subjectNeuroblastomaen_US
dc.subjectMidkineen_US
dc.titleEnhancement of vinorelbine-induced cytotoxicity and apoptosis by clomipramine and lithium chloride in human neuroblastoma cancer cell line SH-SY5Yen_US
dc.typeArticleen_US

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