Aberrant crypt foci are regionally affected by zinc treatment in a 1,2-dimethylhydrazine induced colon carcinogenesis model

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dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorBoumaza, Belkacem Mohamed Amine
dc.contributor.authorFerrat, Meriem
dc.contributor.authorNagy, Andras-Laszlo
dc.contributor.authorOlteanu, Diana Elena
dc.contributor.authorBounaama, Abdelkader
dc.contributor.authorClichici, Simona
dc.date.accessioned2019-10-27T10:46:14Z
dc.date.available2019-10-27T10:46:14Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractZinc is a trace element widely known for its marked antioxidant properties. To gain more insight into the site and time- specific mechanisms by which it induces chemoprevention, this study was elaborated over a pre-cancerous model of colon carcinogenesis. Colon cancer was induced by 1,2-dimethylhydrazine (DMH) in mice (20 mg/kg for 2 weeks) and groups of animals were supplemented with or without zinc sulfate (ZnSO4, 200 mg/L) in drinking water for 4, 10 or 14 weeks. Colon tissues were collected for pathological observation, analyzing aberrant crypt (AC) and aberrant crypt foci (ACF) formations, multiplicity and distribution. Similarly, histological assessment and mucin production, as well as oxidative stress markers estimation was performed for the different groups. Results showed a significant increase in ACF and AC numbers, ACF multiplicity and demonstrated stronger distal occurrence than in the proximal after DHM administration. Histopathological analysis presented marked structural alterations and mucin loss in the distal than the proximal colons. A significant increase in myeloperoxidase (MPO), nitric oxide (NO), L-omithine and malondialdehyde (MDA) levels was observed followed by a significant decrease in antioxidant markers (superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH)). Oral ZnSO4 supplementation (continuous or partial) induced significant decrease in ACF, AC numbers and multiplicity, restored histological architecture and mucin production, and a significant decrease in proinflammatory markers while it reduced antioxidants to normal levels. From this study, insight was obtained on the use of ZnSO4 as a chemopreventive agent and shed light on its potential, as a supplement in nutraceutical approaches.en_US
dc.description.sponsorshipDGRSDT (Direction Generale de la Recherche Scientifique et du Developpement Technologique) Algiers, Algeria (PNR project: ATRSS/PNR 08) [305]; Agence Universitaire de la Francophonie Bureau Europe Centrale et Orientale (AUF-Beco), Romaniaen_US
dc.description.sponsorshipThis work was supported by the DGRSDT (Direction Generale de la Recherche Scientifique et du Developpement Technologique) Algiers, Algeria (PNR project: ATRSS/PNR 08/No305) and the Agence Universitaire de la Francophonie Bureau Europe Centrale et Orientale (AUF-Beco), Romania.en_US
dc.identifier.doi10.1016/j.jtemb.2018.01.009en_US
dc.identifier.endpage30en_US
dc.identifier.issn0946-672X
dc.identifier.pmid29544804en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.1016/j.jtemb.2018.01.009
dc.identifier.urihttps://hdl.handle.net/11454/31271
dc.identifier.volume47en_US
dc.identifier.wosWOS:000429396900003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbh, Urban & Fischer Verlagen_US
dc.relation.ispartofJournal of Trace Elements in Medicine and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectZinc sulfateen_US
dc.subjectColon canceren_US
dc.subjectDistal proximalen_US
dc.subjectDimethylhydrazineen_US
dc.subjectACFen_US
dc.subjectOxidative stressen_US
dc.titleAberrant crypt foci are regionally affected by zinc treatment in a 1,2-dimethylhydrazine induced colon carcinogenesis modelen_US
dc.typeArticleen_US

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