The comparison of apoptosis-related protein expressions in neurotoxin-based in vitroParkinson’s Disease models

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dc.contributor.authorKaftan, Gizem
dc.contributor.authorArmagan, Güliz
dc.contributor.authorDağcı, Taner
dc.date.accessioned2021-05-03T21:16:30Z
dc.date.available2021-05-03T21:16:30Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractProgrammed cell death (apoptosis) is mainly responsible for neuronal damage in neurodegenerative diseases. Thus, inhibition of apoptosis could represent an effective strategy in the prevention of these diseases. In this study, we aimed to compare the apoptotic responses of neurotoxins that are widely used to induce neuronal damage incell culture studies and help to decide the most suitable experimental model for drug studies that target apoptosis. Cell viability analyses were performed by MTT assay following 1-methyl-4-phenylpyridinium (MPP+), 6-hydroxydopamine (6-OHDA), rotenone andparaquat treatments at three different time points(12, 24, 48h). Pro-apoptotic (Bax, Bad, Bak), anti-apoptotic (Bcl-2, Bcl-xl) protein levels and total caspase-3 protein levels were determined by Western Blotting technique following treatments. As expected, all neurotoxins managed to trigger cell death and apoptotic pathway. On the other hand, each neurotoxin was found to enhance and/or reduce the levels of different proteins that are associated with apoptosis. Due to different responses of apoptosis related proteins to neurotoxins, it can be concluded that the determination of target proteins with a number of protein-binding assays prior to cell culture studies and then deciding an in vitro model are essential while screening newly synthesized drugs that target apoptosis.en_US
dc.identifier.doi10.35333/jrp.2020.117
dc.identifier.endpage120en_US
dc.identifier.issn2630-6344
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85079274385en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage112en_US
dc.identifier.trdizinid377862en_US
dc.identifier.urihttps://doi.org/10.35333/jrp.2020.117
dc.identifier.urihttps://hdl.handle.net/11454/71913
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/377862
dc.identifier.volume24en_US
dc.identifier.wosWOS:000508432400010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofJournal of research in pharmacy (online)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keywords]en_US
dc.titleThe comparison of apoptosis-related protein expressions in neurotoxin-based in vitroParkinson’s Disease modelsen_US
dc.typeArticleen_US

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