Cutaneous Leishmaniasis Cases Caused by Leishmania infantum in Sanliurfa Province, Turkey

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dc.contributor.authorZeyrek, Fadile Yildiz
dc.contributor.authorToz, Seray
dc.contributor.authorUluca, Nermin
dc.contributor.authorDoni, Nebiye
dc.contributor.authorToprak, Sahin
dc.contributor.authorOzbel, Yusuf
dc.date.accessioned2020-12-01T11:58:12Z
dc.date.available2020-12-01T11:58:12Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractLeishmaniases are a group of vector-borne diseases, and two clinical forms, visceral (VL) and cutaneous leishmaniasis (CL, Oriental sore), are seen in Turkey. While VL cases are recorded as 20-25 per year, CL cases are reported around 2000 per year, and nearly half of CL cases were recorded in anliurfa province. Therefore, by knowing the epidemiology of the disease in anliurfa province, it is possible to develop control measures and reduce the total number of cases across the country. Although Leishmania tropica is known as the main causative agent in anliurfa, other Leishmania species have also been identified as a result of mass human movements in the last 10 years. in this study, we aimed to present the first CL cases caused by Leishmania infantum in $anllurfa. A total of 14 cases, which were admitted with the suspicion of CL and diagnosed as positive by direct microscopy and/or real-time ITS1-PCR using lesion aspiration samples are included in the study. Two or more smears were prepared from the samples taken from the lesions of the patients by fine needle aspiration. One of the smears was stained with Giemsa stain after fixation with methyl alcohol and examined under the light microscope at x1000 magnification for the presence of Leishmania amastigotes. DNA isolation was made from the other unstained preparations with a commercial kit (Qiagen DNeasy, Germany) according to the recommendations of the manufacturer. the real-time ITS1-PCR method was performed by using the Old World species-specific primers and probes. As a result, by the identification of the species with real-time ITS1-PCR, it was determined that the causative agent was L.infantum in five cases, Lmajor in one case and L.tropica in eight cases. It was learned that four of the cases in which L.infantum was detected as the causative agent were local, one was Syrian and they lived in the city center. Also two of the eight cases, which were identified as L.tropica, were Syrian and six of them were domestic cases and all of them lived in the city center. While all 14 patients included in the study were positive with real-time ITS1-PCR, amastigotes were detected in 10 cases only. the cases of CL presented in this study are the first cases caused by L.infantum reported from anliurfa, and are important in terms of concretely demonstrating the effect of mass human mobility and migration on the epidemiology of the infection.en_US
dc.identifier.doi10.5578/mb.69909en_US
dc.identifier.endpage656en_US
dc.identifier.issn0374-9096
dc.identifier.issue4en_US
dc.identifier.pmid33107294en_US
dc.identifier.scopus2-s2.0-85094868031en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage647en_US
dc.identifier.urihttps://doi.org/10.5578/mb.69909
dc.identifier.urihttps://hdl.handle.net/11454/61950
dc.identifier.volume54en_US
dc.identifier.wosWOS:000582397200013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCutaneous leishmaniasisen_US
dc.subjectLeishmania infantumen_US
dc.subjectLeishmania tropicaen_US
dc.subjectLeishmania majoren_US
dc.subjectSanliurfaen_US
dc.subjectTurkeyen_US
dc.titleCutaneous Leishmaniasis Cases Caused by Leishmania infantum in Sanliurfa Province, Turkeyen_US
dc.typeArticleen_US

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