Electrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseases

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dc.contributor.authorKara, Pinar
dc.contributor.authorCavdar, Seda
dc.contributor.authorMeric, Buket
dc.contributor.authorErensoy, Selda
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T19:59:15Z
dc.date.available2019-10-27T19:59:15Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractDirect electrochemical genosensor was developed for the detection of a probe sequence relative position in a PCR amplicon for the optimum detection of bacterial and microbiological diseases, in this study. The genosensor relies on a label-free electrochemical detection. The amino-linked inosine modified (guanine-free) coequal capture probes which were chosen from different parts of a PCR amplicon, immobilized on to disposable pencil graphite electrodes (PGE) by electrostatically and covalently. As a model case Hepatitis B virus (HBV) genome amplicon was used for the detection and specification. Hybridization was occurred after surface coverage with denatured amplicons. After hybridization, optimum probe sequence position was identified by using the differences between the responses of guanine oxidation signals. The results of this study might have a great convenience for the microbiological diseases detection applications such as DNA micro arrays. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.bioelechem.2007.05.001en_US
dc.identifier.endpage210en_US
dc.identifier.issn1567-5394
dc.identifier.issue2en_US
dc.identifier.pmid17604234en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage204en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2007.05.001
dc.identifier.urihttps://hdl.handle.net/11454/41162
dc.identifier.volume71en_US
dc.identifier.wosWOS:000251406800015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectelectrochemical genosensoren_US
dc.subjectDNAen_US
dc.subjectPCRen_US
dc.titleElectrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseasesen_US
dc.typeArticleen_US

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