Imaging and detection of cell apoptosis by In vitro photodynamic therapy applications of zinc (II) phthalocyanine on human melanoma cancer

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dc.authoridYurt, Fatma/0000-0002-9394-6908
dc.authorscopusid57253183500
dc.authorscopusid57200179167
dc.authorscopusid56998644400
dc.authorscopusid6602719646
dc.authorwosidYÜZER, Abdulcelil/AAX-4968-2021
dc.contributor.authorZeinali, Sevda
dc.contributor.authorTuncel, Ayca
dc.contributor.authorYuzer, Abdulcelil
dc.contributor.authorYurt, Fatma
dc.date.accessioned2023-01-12T19:56:05Z
dc.date.available2023-01-12T19:56:05Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractThis study aims to investigate the photodynamic therapy (PDT) effects on MeWo (human melanoma cells) and HaCaT (normal human keratinocyte cells) by light stimulation of different concentrations of Zinc (II)-tetra-tertbutyl-phthalocyaninato (ZnPc). MTT viability assay data indicated that a 25 mu M concentration of ZnPc is cytotoxic to the melanoma cancer cells while this concentration of ZnPc is not cytotoxic for the HaCaT cell line. Moreover, the results showed that photoactivated ZnPc at 12.5 mu M concentration reduced the cell viability of the MeWo cell line to about 50 %. At this photosensitizing concentration, the efficacy of light doses of 20, 30, 40, and 50 J/cm(2) was evaluated against MeWo and HaCaT cells. ZnPc at a concentration of 12.5 mu M activated with a light dose of 50 J/cm(2) was the most efficient for the killing of MeWo cells. In conclusion, the 12.5 mu M of ZnPc with the treatment light dose of 50 J/cm(2) from a RED light source was adequate to destroy MeWo cells by the ROS-induced apoptosis mechanism. It also exhibited low killing effects on healthy HaCaT cells. These findings are supported by the results of apoptosis with the Annexin V & Dead Cell Kit and fluorescence imaging.en_US
dc.identifier.doi10.1016/j.pdpdt.2021.102518
dc.identifier.issn1572-1000
dc.identifier.issn1873-1597
dc.identifier.pmid34478898en_US
dc.identifier.scopus2-s2.0-85114610592en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.pdpdt.2021.102518
dc.identifier.urihttps://hdl.handle.net/11454/76800
dc.identifier.volume36en_US
dc.identifier.wosWOS:000709143800002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhotodynamic therapyen_US
dc.subjectMelanomaen_US
dc.subjectZinc phthalocyanineen_US
dc.subjectHuman melanoma cellsen_US
dc.subjectNormal human keratinocyte cellsen_US
dc.subjectPdt Activitiesen_US
dc.subjectPhotosensitizersen_US
dc.subjectPathwaysen_US
dc.subjectEfficacyen_US
dc.titleImaging and detection of cell apoptosis by In vitro photodynamic therapy applications of zinc (II) phthalocyanine on human melanoma canceren_US
dc.typeArticleen_US

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