Protective effect of edaravone against manganese-induced toxicity in cultured rat astrocytes

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dc.contributor.authorEvren V.
dc.contributor.authorApaydin M.
dc.contributor.authorKhalilnezhad A.
dc.contributor.authorErbas O.
dc.contributor.authorTaskiran D.
dc.date.accessioned2019-10-26T21:21:46Z
dc.date.available2019-10-26T21:21:46Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractManganese (Mn), a trace metal, is essential for maintaining the normal regulation of many biochemical and cellular processes. However, accumulation of Mn due to excessive environmental exposure leads to neurological impairment, referred to as manganism. Edaravone (EDA) is a potent free radical scavenger that has been clinically shown to reduce the neuronal injury after cerebral ischemia. In the present study, we aimed to examine the protective effects of EDA against Mn toxicity in astrocyte cultures. Astrocyte cultures were prepared from cerebral cortices of newborn Sprague-Dawley rats. The experiments were performed between 16 and 18 days of cultures. Astrocytes were treated in DMEM medium containing Mn (1-1000. µM) for 24. h to test Mn toxicity. In order to assess the effect of EDA, cells were pre-treated with different doses of EDA (10, 100 and 1000. µM) 6. h before Mn treatment. Cell viability (MTT), apoptotic cell death (Hoechst test) and lipid peroxide levels were evaluated in cultures. Our results showed that Mn significantly and dose-dependently reduced cell viability in astrocyte cultures. The apoptotic cell death and lipid peroxides were significantly higher in Mn treated cultures. Treatment of astrocytes with EDA successfully suppressed oxidative stress and cell death due to Mn exposure. The findings of the present study suggest that Mn cytotoxicity is mainly associated with ROS generation and apoptotic cell death. Besides, EDA may have beneficial effects against Mn toxicity. However, further studies are needed to elucidate the molecular mechanisms underlying protective effect of EDA. © 2015 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.etap.2015.08.010en_US
dc.identifier.endpage567en_US
dc.identifier.issn1382-6689
dc.identifier.issue2en_US
dc.identifier.pmid26335034en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage563en_US
dc.identifier.urihttps://doi.org/10.1016/j.etap.2015.08.010
dc.identifier.urihttps://hdl.handle.net/11454/16907
dc.identifier.volume40en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofEnvironmental Toxicology and Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAstrocyteen_US
dc.subjectEdaravoneen_US
dc.subjectManganeseen_US
dc.subjectOxidative stressen_US
dc.subjectToxicityen_US
dc.titleProtective effect of edaravone against manganese-induced toxicity in cultured rat astrocytesen_US
dc.typeArticleen_US

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