Bioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoa

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dc.contributor.authorErel, Sura Baykan
dc.contributor.authorDemir, Serdar
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorBallar, Petek
dc.contributor.authorKhan, Shabana
dc.contributor.authorYavasoglu, N. Ulku Karabay
dc.contributor.authorKaraalp, Canan
dc.date.accessioned2019-10-27T22:14:54Z
dc.date.available2019-10-27T22:14:54Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractContext: Centaurea L. (Asteraceae) species used as herbal remedies in Turkish traditional medicine have shown several biological properties. Objective: Extracts obtained from the aerial parts of Centaurea aphrodisea Boiss., Centaurea athoa DC., Centaurea hyalolepis Boiss., Centaurea iberica Trev. and Centaurea polyclada DC. were evaluated for their antioxidant, cytotoxic and anti-inflammatory activities. Materials and methods: Extracts of Centaurea species were tested for their antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) screening assays and for in vitro anti-inflammatory activity by Nf-kappa B and iNOS inhibition assays. The extracts were tested for their in vitro cytotoxicities against a panel of human solid tumor cell lines (SK-MEL: malignant melanoma, KB: oral epidermal carcinoma, BT-549: breast ductal carcinoma and SK-OV-3: ovary carcinoma) as well as non-cancerous kidney fibroblast (Vero) and kidney epithelial cells (LLC-PK1) by Neutral Red assay. In vivo anti-inflammatory activity of C. athoa was evaluated by the carrageenan-induced paw edema test in rats. Results: Antioxidant activities were observed for methanol extracts of plants. C. polyclada had the strongest effect on BT-549, KB and SK-OV-3 cell lines (30, 33 and 47 mu g/ml, respectively). Nf-kappa B inhibition of chloroform extract of C. athoa was determined equivalent to positive control parthenolide (IC50: 6 mu g/ml). This extract also showed anti-inflammatory activity by the carrageenan-induced paw edema test in rats, in all hours at a dose of 50 mg/kg compared to the control group. Discussion and conclusion: C. athoa is suggested to be a potential source of lead compounds for inflammatory diseases due to the significant in vitro and in vivo anti-inflammatory results.en_US
dc.identifier.doi10.3109/13880209.2013.868493en_US
dc.identifier.endpage781en_US
dc.identifier.issn1388-0209
dc.identifier.issn1744-5116
dc.identifier.issue6en_US
dc.identifier.pmid24405079en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage775en_US
dc.identifier.urihttps://doi.org/10.3109/13880209.2013.868493
dc.identifier.urihttps://hdl.handle.net/11454/50088
dc.identifier.volume52en_US
dc.identifier.wosWOS:000336506400017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofPharmaceutical Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntioxidanten_US
dc.subjectCentaurea aphrodiseaen_US
dc.subjectCentaurea athoaen_US
dc.subjectCentaurea hyalolepisen_US
dc.subjectCentaurea ibericaen_US
dc.subjectCentaurea polycladaen_US
dc.subjectcytotoxicityen_US
dc.titleBioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoaen_US
dc.typeArticleen_US

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