Chitosan-ferrocene film as a platform for flow injection analysis applications of glucose oxidase and Gluconobacter oxydans biosensors

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dc.contributor.authorYilmaz, Ovgu
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorGulcemal, Suleyman
dc.contributor.authorKilinc, Ali
dc.contributor.authorTimur, Suna
dc.contributor.authorCetinkaya, Bekir
dc.date.accessioned2019-10-27T21:40:47Z
dc.date.available2019-10-27T21:40:47Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractChitosan-ferrocene (CHIT-Fc) hybrid was synthesized through covalent modification and its electrochemical properties in immobilized form were studied by using cyclic voltammetry. The hybrid film exhibited reversible electrochemistry with a formal potential of +0.35V (vs. Ag/AgCl) at pH 5.5. The Fc in CHIT matrix retained its electrocatalytic activity and did not diffuse from the matrix. This redox-active hybrid was further employed as a support for immobilization of glucose oxidase (GOx) and whole cells of Gluconobacter oxydans using glutaraldehyde on a glassy carbon electrode (GCE). The experimental conditions were optimized and the analytical characteristics of enzyme and microbial biosensors were evaluated for glucose in flow injection analysis (FIA) system. Under optimized conditions, both enzyme and microbial biosensors exhibited wide linear ranges for glucose from 2.0 to 16.0 mM and from 1.5 to 25.0 mM, respectively. Moreover, the biosensors have the advantages of relatively fast response times, good reproducibility and stability in FI mode. It was demonstrated that CHIT-Fc provides a biocompatible microenvironment for both bioctalysts and an electron transfer pathway. Additionally, integration of the enzyme and microbial biosensors into the FIA system has several advantages including capability of automation and high throughput at low cost. This promising redox hybrid can be utilized as an immobilization matrix for biomolecules in biosensor systems. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.colsurfb.2012.05.020en_US
dc.identifier.endpage68en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid22766283en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage62en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2012.05.020
dc.identifier.urihttps://hdl.handle.net/11454/46370
dc.identifier.volume100en_US
dc.identifier.wosWOS:000307683900009en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectFIAen_US
dc.subjectFerroceneen_US
dc.subjectChitosanen_US
dc.subjectSurface modificationen_US
dc.titleChitosan-ferrocene film as a platform for flow injection analysis applications of glucose oxidase and Gluconobacter oxydans biosensorsen_US
dc.typeArticleen_US

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