Confirmation of the presence of Mycobacterium tuberculosis and other mycobacteria in mycobacterial growth indicator tubes (MGIT) by multiplex strand displacement amplification

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dc.contributor.authorBadak F.Z.
dc.contributor.authorKiska D.L.
dc.contributor.authorO'Connell M.
dc.contributor.authorNycz C.M.
dc.contributor.authorHartley C.
dc.contributor.authorSetterquist S.
dc.contributor.authorHopfer R.L.
dc.date.accessioned2019-10-27T00:32:19Z
dc.date.available2019-10-27T00:32:19Z
dc.date.issued1997
dc.departmentEge Üniversitesien_US
dc.description.abstractMultiplex strand displacement amplification (mSDA) is capable of amplifying three distinct DNA sequences simultaneously. These include sequences present in most genera of mycobacteria, a sequence specific for Mycobacterium tuberculosis, and an internal control. mSDA was used to detect the presence of these target sequences in 154 (72 positive, 76 negative, and 6 failed) clinical specimens cultured in the mycobacterial growth indicator tube (MGIT) system. A wide variety of specimen types were processed and cultured. Once these cultures were deemed positive by MGIT fluorescence or were deemed negative after 8 weeks of incubation, MGIT culture aliquots were processed for mSDA analyses. A chemiluminescent microwell assay was used to detect the amplified products. The procedure was relatively simple and took less than 6 h to complete. The sensitivity of mSDA for detecting acid-fast bacilli was 96.4% compared to that of MGIT culture. Sensitivity and specificity were 97.2 and 96.1%, respectively, when all clinical criteria were considered. mSDA was shown to be a rapid and effective method for confirming the presence of M. tuberculosis and other mycobacteria in positive MGIT cultures.en_US
dc.identifier.endpage1243en_US
dc.identifier.issn0095-1137
dc.identifier.issue5en_US
dc.identifier.pmid9114414en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1239en_US
dc.identifier.urihttps://hdl.handle.net/11454/23747
dc.identifier.volume35en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleConfirmation of the presence of Mycobacterium tuberculosis and other mycobacteria in mycobacterial growth indicator tubes (MGIT) by multiplex strand displacement amplificationen_US
dc.typeArticleen_US

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