A biosensor based on urate oxidase-peroxidase coupled enzyme system for uric acid determination in urine

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dc.contributor.authorAkyilmaz, E
dc.contributor.authorSezginturk, MK
dc.contributor.authorDinckaya, E
dc.date.accessioned2019-10-27T18:59:01Z
dc.date.available2019-10-27T18:59:01Z
dc.date.issued2003
dc.departmentEge Üniversitesien_US
dc.description.abstractA new amperometric biosensor based on urate oxidase-peroxidase coupled enzyme system for the specific and selective determination of uric acid in urine was developed. Commercially available urate oxidase and peroxidase were immobilized with gelatin by using glutaraldehyde and fixed on a pretreated teflon membrane. The method is based on generation of H2O2 from urine uric acid by urate oxidase and its consuming by peroxidase and then measurement of the decreasing of dissolved oxygen concentration by the biosensor. The biosensor response depends linearly on uric acid concentration between 0.1 and 0.5 muM. In the optimization studies of the biosensor, phosphate buffer (pH 7.5; 50 mM) and 35 degreesC were obtained as the optimum working conditions. In addition, the most suitable enzyme activities were found as 64.9 x 10(-3) U cm(-2) for urate oxidase and 512.7 U cm(-2) for peroxidase. And also some characteristic studies of the biosensor such as reproducibility, substrate specificity and storage stability were carried out. (C) 2003 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.doi10.1016/S0039-9140(03)00239-Xen_US
dc.identifier.endpage79en_US
dc.identifier.issn0039-9140
dc.identifier.issue2en_US
dc.identifier.pmid18969164en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage73en_US
dc.identifier.urihttps://doi.org/10.1016/S0039-9140(03)00239-X
dc.identifier.urihttps://hdl.handle.net/11454/37574
dc.identifier.volume61en_US
dc.identifier.wosWOS:000186127200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecturic aciden_US
dc.subjecturate oxidaseen_US
dc.subjectperoxidaseen_US
dc.subjectcoupled enzyme systemsen_US
dc.subjectbiosensorsen_US
dc.titleA biosensor based on urate oxidase-peroxidase coupled enzyme system for uric acid determination in urineen_US
dc.typeArticleen_US

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