Transcriptome-Wide Analysis Reveals the Molecular Mechanism of Tumoricidal Effects of Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), on MCF-7 Breast Cancer Cells

Basit Öğe Kaydı
dc.contributor.authorAtay, Sevcan
dc.contributor.authorAk, Handan
dc.contributor.authorKalmis, Erbil
dc.contributor.authorKayalar, Husniye
dc.contributor.authorAydin, Hikmet Hakan
dc.date.accessioned2021-05-03T20:33:25Z
dc.date.available2021-05-03T20:33:25Z
dc.date.issued2021
dc.departmentEge Üniversitesien_US
dc.description.abstractHericium erinaceus (Hericiaceae, Agaricomycetes) is an edible and medicinal mushroom shown to exhibit anticancer effects in various cancers. However, little is known about the effects of H. erinaceus on breast cancer. Here, we aimed to examine the anticancer effects of H. erinaceus water extract on cell viability, apoptosis, and cell cycle distribution in MCF-7 estrogen receptor-positive (ER+) human breast adenocarcinoma cells. We also investigated the possible synergistic interactions between the extract and a known ER antagonist, tamoxifen. Finally, we performed a whole genome transcriptome analysis to investigate the molecular mechanisms underlying the tumoricidal effect of H. erinaceus on breast cancer cells. H. erinaceus water extract was capable of inhibiting cell viability of MCF-7 breast cancer cells in a time- and dose-dependent manner. Treatment with the extract significantly induced apoptosis and G1 cell cycle arrest in cells, consistent with the results of the cell viability analysis. H. erinaceus water extract showed strong synergism with tamoxifen in inhibiting cell viability of MCF-7 cells. The treatment altered the expression of a total of 362 transcripts, including c-Fos, FBJ murine osteosarcoma viral oncogene homolog (FOS), eukaryotic translation initiation factor 1 (EIF1), HES family basic helix-loop-helix transcription factor 1 (HES1), early growth response 2 (EGR2), cyclin L1 (CCNL1), EGR3, Kelch-like family member 24 (KLHL24), and inhibitor of DNA binding 2 (ID2). Results of Gene Ontology enrichment analysis suggest that these genes are mainly associated with transcriptional regulation processes. The cancer signaling pathways that were significantly enriched for differentially expressed genes in response to H. erinaceus treatment were tumor protein P53, Janus kinase-signal transducer and activator of transcription JAK-STAT, transforming growth factor-beta, and mitogen-activated protein kinase. Overall, our results provided in vitro evidence that H. erinaceus water extract could be a potent candidate for the treatment of ER+ breast cancer.en_US
dc.description.sponsorshipEge University Research Grand Fund [2012-TIP-40]en_US
dc.description.sponsorshipThis study was supported by the Ege University Research Grand Fund (2012-TIP-40).en_US
dc.identifier.endpage106en_US
dc.identifier.issn1521-9437
dc.identifier.issn1940-4344
dc.identifier.issn1521-9437en_US
dc.identifier.issn1940-4344en_US
dc.identifier.issue1en_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://hdl.handle.net/11454/70018
dc.identifier.volume23en_US
dc.identifier.wosWOS:000614768900010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherBegell House Incen_US
dc.relation.ispartofInternational Journal of Medicinal Mushroomsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHericium erinaceusen_US
dc.subjectapoptosisen_US
dc.subjectcell cycleen_US
dc.subjectgene expressionen_US
dc.subjectbreast canceren_US
dc.subjectmedicinal mushroomsen_US
dc.titleTranscriptome-Wide Analysis Reveals the Molecular Mechanism of Tumoricidal Effects of Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), on MCF-7 Breast Cancer Cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu