Transcriptome-Wide Analysis Reveals the Molecular Mechanism of Tumoricidal Effects of Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), on MCF-7 Breast Cancer Cells
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Begell House Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Hericium erinaceus (Hericiaceae, Agaricomycetes) is an edible and medicinal mushroom shown to exhibit anticancer effects in various cancers. However, little is known about the effects of H. erinaceus on breast cancer. Here, we aimed to examine the anticancer effects of H. erinaceus water extract on cell viability, apoptosis, and cell cycle distribution in MCF-7 estrogen receptor-positive (ER+) human breast adenocarcinoma cells. We also investigated the possible synergistic interactions between the extract and a known ER antagonist, tamoxifen. Finally, we performed a whole genome transcriptome analysis to investigate the molecular mechanisms underlying the tumoricidal effect of H. erinaceus on breast cancer cells. H. erinaceus water extract was capable of inhibiting cell viability of MCF-7 breast cancer cells in a time- and dose-dependent manner. Treatment with the extract significantly induced apoptosis and G1 cell cycle arrest in cells, consistent with the results of the cell viability analysis. H. erinaceus water extract showed strong synergism with tamoxifen in inhibiting cell viability of MCF-7 cells. The treatment altered the expression of a total of 362 transcripts, including c-Fos, FBJ murine osteosarcoma viral oncogene homolog (FOS), eukaryotic translation initiation factor 1 (EIF1), HES family basic helix-loop-helix transcription factor 1 (HES1), early growth response 2 (EGR2), cyclin L1 (CCNL1), EGR3, Kelch-like family member 24 (KLHL24), and inhibitor of DNA binding 2 (ID2). Results of Gene Ontology enrichment analysis suggest that these genes are mainly associated with transcriptional regulation processes. The cancer signaling pathways that were significantly enriched for differentially expressed genes in response to H. erinaceus treatment were tumor protein P53, Janus kinase-signal transducer and activator of transcription JAK-STAT, transforming growth factor-beta, and mitogen-activated protein kinase. Overall, our results provided in vitro evidence that H. erinaceus water extract could be a potent candidate for the treatment of ER+ breast cancer.
Açıklama
Anahtar Kelimeler
Hericium erinaceus, apoptosis, cell cycle, gene expression, breast cancer, medicinal mushrooms
Kaynak
International Journal of Medicinal Mushrooms
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
23
Sayı
1
