Investigation of In vitro Propagation Possibilities of Endemic Campanula phitosiana Yıldırım & Şentürk

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dc.contributor.authorPirhan, Ademi Fahri
dc.contributor.authorSevindik, Ebru
dc.contributor.authorSevindik, Başar
dc.date.accessioned2024-08-31T07:31:12Z
dc.date.available2024-08-31T07:31:12Z
dc.date.issued2022
dc.departmentEge Üniversitesien_US
dc.description.abstractCampanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia (both İzmir and Aydın). This species belongs to the Mediterranean basin floristic region. According to the International Union for Conservation of Nature’s criteria, C. phitosiana is classified as “Critically Endangered” (CR). The aim of the study is to develop in vitro regeneration protocol for critically endangered endemic Campanula phitosiana. To investigate the efficient medium and plant growth regulator combinations for callus initiation and shoot proliferation, petiole and leaves were used as explant and explants cultivated on MS medium including NAA (1-Naphthylacetic acid) (0.3 mg L-1), TDZ (Thidiazuron) (0.5, 1.0, 2.0, and 3.0 mg L-1), BA (6-Benzylaminopurine) (0.5, 1.0, 2.0, and 3.0 mg L-1) and Gibberellic Acid (GA3) (0.5, 1.0, 2.0, and 3.0 mg L-1). Explants subcultured 3 times. Experiments were conducted according to completely randomized design repeated with 5 replicates and each replicates including 5 explants. As a result of the experiment, callus initiation and shoot proliferation were investigated. Efficient callus initiation was observed petiole explants as 100% rate. Shoot proliferation was observed on MS medium including 0.3 mg L-1 NAA + 2.0 mg L-1 GA3.en_US
dc.identifier.doi10.16882/HortiS.1200592
dc.identifier.endpage112en_US
dc.identifier.issn2717-882X
dc.identifier.issn2149-2182
dc.identifier.issue3en_US
dc.identifier.startpage107en_US
dc.identifier.trdizinid1173914en_US
dc.identifier.urihttps://doi.org/10.16882/HortiS.1200592
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1173914
dc.identifier.urihttps://hdl.handle.net/11454/103358
dc.identifier.volume39en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHorticultural Studiesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.titleInvestigation of In vitro Propagation Possibilities of Endemic Campanula phitosiana Yıldırım & Şentürken_US
dc.typeArticleen_US

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