Antibody separation using lectin modified poly(HEMA-EDMA) hydrogel membranes

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dc.contributor.authorDemir, Esra Feyzioglu
dc.contributor.authorKuru, Cansu Ilke
dc.contributor.authorUygun, Murat
dc.contributor.authorUygun, Deniz Aktas
dc.contributor.authorAkgol, Sinan
dc.date.accessioned2019-10-27T10:44:32Z
dc.date.available2019-10-27T10:44:32Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractHerein we describe the synthesis of Concanavalin A-poly(2-hydroxyethyl methacrylate-ethylene dimethacrylate) hydrogel membranes (via photopolymerization technique) for antibody separation from aqueous solutions. Different characterization techniques including Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, Elemental Analysis and swelling tests revealed the highly rough morphology and spherical shape of the synthetized membranes. Attached amount of IMEO (salinization agent) onto polymeric structure and Con A binding capacity were found to be 10.85 mol/g and 3.52 mg/g, respectively. Optimum conditions for IgG adsorption such as adsorption capacity, pH and reusability profile of HMs were judiciously characterized. Maximum IgG adsorption capacity of hydrogel membrane was found to be as 26.81 mg/g. Adsorbed IgG was eluted successfully by using 2.0 M of NaCl solution. Reusability profiles of hydrogel membrane in five adsorption-desorption cycles revealed that there was no significant decrease in IgG adsorption capacity at the end of the 5th reuse. The hydrogel membranes reported here hold considerable promise as an effective sorbent system for IgG adsorption with good stability and efficient repeated usage.en_US
dc.identifier.doi10.1080/09205063.2017.1417197en_US
dc.identifier.endpage359en_US
dc.identifier.issn0920-5063
dc.identifier.issn1568-5624
dc.identifier.issue4en_US
dc.identifier.pmid29241441en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage344en_US
dc.identifier.urihttps://doi.org/10.1080/09205063.2017.1417197
dc.identifier.urihttps://hdl.handle.net/11454/30995
dc.identifier.volume29en_US
dc.identifier.wosWOS:000424795200002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of Biomaterials Science-Polymer Editionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLectin affinity chromatographyen_US
dc.subjecthydrogel membranesen_US
dc.subjectsilanizationen_US
dc.subjectconcanavalin Aen_US
dc.subjectIgG adsorptionen_US
dc.titleAntibody separation using lectin modified poly(HEMA-EDMA) hydrogel membranesen_US
dc.typeArticleen_US

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