Effect of apoptosis and response of extracellular matrix proteins after chemotherapy application on human breast cancer cell spheroids

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dc.contributor.authorOktem, G
dc.contributor.authorVatansever, S
dc.contributor.authorAyla, S
dc.contributor.authorUysal, A
dc.contributor.authorAktas, S
dc.contributor.authorKarabulut, B
dc.contributor.authorBilir, A
dc.date.accessioned2019-10-27T19:19:55Z
dc.date.available2019-10-27T19:19:55Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractMulticellular Tumor Spheroid (NITS) represents a three-dimentional structural form of tumors in laboratory conditions, and it has the characteristics of avascular micrometastases or intervascular spaces of big tumors. Recent studies indicate that extracellular matrix (ECM) proteins play a critical role in tumor metastasis, therefore normal and cancer cells require an ECM for survival, proliferation and differentiation. Doxorubicin and Docetaxel are widely used in the therapy of breast cancer, as well as in in vivo and in vitro studies. In this study, we examined the effect of apoptosis and proliferation of cells on the human breast cancer cell line, MCF-7, by using p53, bcl-2 and Ki67 gene expression, and the tendency to metastasis with extracellular matrix proteins, laminin and type IV collagen after chemotherapy in the spheroid model. The apoptotic cell death in situ was detected by TUNEL method. TUNEL-positive cells and positive immunoreactivities of laminin, type IV collagen, p53 and, bel-2 were detected in the control group. There was no laminin and type IV collagen immunoreactivities in spheroids of drug groups. While TUNEL-positive cells and p53 immunoreactivity were detected in Docetaxel, Doxorubicin and Docetaxel/Doxorubicin groups, p53 immunoreactivity was not observed in the Docetaxel group. There was no bcl-2 immunoreactivity in either drug group. In addition, we did not detect Ki67 immunoreactivity in both control and drug treatment groups. However, the absence of Ki67 protein in MCF-7 breast multicellular tumor spheroids is possibly related to the cells in GO or S phase. These agents may affect the presence of ECM proteins in this in vitro model of micrometastasis of spheroids. These findings suggest that the possible mechanism of cell death in Doxorubicin and Docetaxel/Doxorubicin treatment groups is related to apoptosis through the p53 pathway. However, we considered the possiblity that there is another control mechanism for the Docetaxel group.en_US
dc.identifier.endpage340en_US
dc.identifier.issn1021-335X
dc.identifier.issue2en_US
dc.identifier.pmid16391851en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage335en_US
dc.identifier.urihttps://hdl.handle.net/11454/38810
dc.identifier.volume15en_US
dc.identifier.wosWOS:000234781800008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherProfessor D A Spandidosen_US
dc.relation.ispartofOncology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectspheroiden_US
dc.subjectchemotherapyen_US
dc.subjectapoptosisen_US
dc.subjectECM proteinsen_US
dc.titleEffect of apoptosis and response of extracellular matrix proteins after chemotherapy application on human breast cancer cell spheroidsen_US
dc.typeArticleen_US

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