The role of nitric oxide in proteoglycan turnover by bovine articular cartilage organ cultures

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dc.contributor.authorStefanovic-Racic M.
dc.contributor.authorMorales T.I.
dc.contributor.authorTaskiran D.
dc.contributor.authorMcIntyre L.A.
dc.contributor.authorEvans C.H.
dc.date.accessioned2019-10-27T00:32:48Z
dc.date.available2019-10-27T00:32:48Z
dc.date.issued1996
dc.departmentEge Üniversitesien_US
dc.description.abstractMonolayer cultures of articular chondrocytes synthesize large amounts of nitric oxide (NO) following exposure to IL-1. The latter has antianabolic and procatabolic activities on these cells, but little is known about the role, if any, of NO in the integrated metabolic pathways of the chondrocyte. In the present study, the role of endogenously produced NO in both the synthesis and degradation of proteoglycans was investigated for the first time. Bovine articular cartilage slices exposed to 20 U/ml human rIL-1ß (hrIL-1ß) synthesized large amounts of NO for 1 to 2 days, after which production fell to a steady state level ~20% of the peak value for the remainder of the 14- day incubation. The NO synthase inhibitor, N-monomethyl L-arginine (L-NMA, 1 mM), blocked NO production and enhanced the acute catabolic effects of hrIL- 1ß in cartilage derived from both calves and adult animals. However, in late cultures, release of proteoglycans was reduced in the presence of L-NMA. The proteolytic activity in conditioned medium of these cultures (measured as caseinolytic activity) was enhanced by L-NMA; however, this inhibitor did not affect the rates of synthesis of proteoglycans. Although NO is widely assumed to be a mediator of cartilage catabolism, our data suggest that it may instead have an acute protective effect. Whether this effect is maintained chronically is less clear.en_US
dc.identifier.endpage1220en_US
dc.identifier.issn0022-1767
dc.identifier.issue3en_US
dc.identifier.pmid8558000en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1213en_US
dc.identifier.urihttps://hdl.handle.net/11454/23897
dc.identifier.volume156en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleThe role of nitric oxide in proteoglycan turnover by bovine articular cartilage organ culturesen_US
dc.typeArticleen_US

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