Design of Tc-99m-DTPA-CLP and Preliminary Evaluation in Rats
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Radiopharmaceuticals are localized in (malignant) tumor tissues by different mechanisms. One of these mechanisms, gelatinase enzyme activity, is associated with poor prognosis in cancer patients and potential targets for tumor imaging. There are some gelatinases to be associated with metastatic potential for tumor imaging to possibly predict metastases. In this study, a cyclic decapeptide conjugate, DTPA-CLP (DTPA-Cys-Leu-Pro-Gly-His-Trp-Gly-Phe-Pro-Ser-Cys), was selected as a peptide conjugate because of its selective inhibitory activity toward gelatinases. Peptide-conjugated DTPA-CLP was labeled with Tc-99m with a radiolabeling efficiency of 97.0 +/- 2.8%. After determining optimization conditions for radiolabeling, a biodistribution study of radiolabeled peptide in albino Wistar rats was performed. According to biodistribution data, Tc-99m-DTPA-CLP showed high uptake in the lung, liver, uterus, and spleen. These results show that Tc-99m-DTPA-CLP may be used for the imaging of gelatinase activity in metastatic tumors.
Açıklama
Anahtar Kelimeler
DTPA-CLP, gelatinase, HWGF-containing peptide conjugate, matrix metalloproteinases, radiolabeled peptide
Kaynak
Chemical Biology & Drug Design
WoS Q Değeri
Q2
Scopus Q Değeri
Cilt
83
Sayı
3
