Polypeptide with electroactive endgroups as sensing platform for the abused drug 'methamphetamine' by bioelectrochemical method

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dc.contributor.authorDemir, Bilal
dc.contributor.authorYilmaz, Tulay
dc.contributor.authorGuler, Emine
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorAkbulut, Huseyin
dc.contributor.authorAldemir, Ebru
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorColak, Demet Goen
dc.contributor.authorCianga, Ioan
dc.contributor.authorYamada, Shuhei
dc.contributor.authorTimur, Suna
dc.contributor.authorEndo, Takeshi
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-10-27T11:24:17Z
dc.date.available2019-10-27T11:24:17Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractAffinity-type sensors have emerged as outstanding platforms in the detection of diagnostic protein markers, nucleic acids and drugs. Thus, these novel platforms containing antibodies could be integrated into the monitoring systems for abused drugs. Herein, we established a novel detection platform for the analysis of a common illicit drug; methamphetamine (METH). Initially, a fluorescent-labeled polypeptide (EDOT-BTDA-Pala), derived from L-alanine N-carboxyanhydride (L-Ala-NCA) via ring-opening polymerization using 4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)benzo[c][1,2,5]thiadiazole-5,6-diamine (EDOT-NH2-BTDA) as initiator, was employed as a glassy carbon electrode (GCE) covering host, in order to immobilize the METH selective antibody. Prior to the examination of analytical features, GCE/EDOT-BTDA-Pala/Antibody surface was successfully characterized in the way of electrochemical (cyclic voltammetry and electrochemical impedance spectroscopy) and microscopic techniques (scanning electron microscopy and fluorescence microscopy). As for the analytical characterization, linearity and limit of detection (LOD) were found as 10-100 mu g/mL with an equation of y=0.0429x-0.2347, (R-2=0.996) and 13.07 mu g/mL, respectively. Moreover, sample application using artificial urine, saliva and serum samples spiked with METH (10, 25, 50 mu g/mL) were performed and LC-MS/MS system was used for further confirmation. The described platform can be adapted to monitor the other types of abused drugs by using suitably selected biorecognition elements.en_US
dc.description.sponsorshipIndustrial Thesis Support Program (SAN-TEZ) of Republic of TurkeyMinistry of Science, Industry & Technology - Turkey; Ministry of Science, Industry and Technology [0620.STZ.2014]; Republic of Turkey, Ministry of DevelopmentTurkiye Cumhuriyeti Kalkinma Bakanligi [2016K121190]; Ege University Research Fund through BAP project [16-FEN-004]; Istanbul Technical University Research Fund; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis work was supported by Industrial Thesis Support Program (SAN-TEZ) of Republic of Turkey, Ministry of Science, Industry and Technology (Project Grant No: 0620.STZ.2014) and Republic of Turkey, Ministry of Development (Project Grant No: 2016K121190). This work was also partially supported by Ege University Research Fund through BAP project (Project No: 16-FEN-004) and Istanbul Technical University Research Fund. G. Bozokalfa from Ege University is acknowledged for the technical helps. Likrom Company was acknowledged for their support to the chromatographic analyses. One of the authors (I.C) thanks to TUBITAK for the financial support by means of "Fellowships For Visiting Scientists and Scientists On Sabbatical Leave" programme.en_US
dc.identifier.doi10.1016/j.talanta.2016.09.042en_US
dc.identifier.endpage796en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid27769482en_US
dc.identifier.startpage789en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2016.09.042
dc.identifier.urihttps://hdl.handle.net/11454/33261
dc.identifier.volume161en_US
dc.identifier.wosWOS:000386989500104en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolypeptideen_US
dc.subjectSurface modificationen_US
dc.subjectAbused drug analysisen_US
dc.subjectMethamphetamineen_US
dc.subjectImmunosensoren_US
dc.subjectChromatographyen_US
dc.titlePolypeptide with electroactive endgroups as sensing platform for the abused drug 'methamphetamine' by bioelectrochemical methoden_US
dc.typeArticleen_US

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