Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein

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dc.contributor.authorZencir, Sevil
dc.contributor.authorOvee, Mohiuddin
dc.contributor.authorDobson, Melanie J.
dc.contributor.authorBanerjee, Monimoy
dc.contributor.authorTopcu, Zeki
dc.contributor.authorMohanty, Smita
dc.date.accessioned2019-10-27T21:37:01Z
dc.date.available2019-10-27T21:37:01Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractThe vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting protein (GIP), also known as Tax Interacting Protein TIP-I, is unique in being composed almost exclusively of a single PDZ domain. GIP has important roles in cellular signaling, protein scaffolding and modulation of tumor growth and interacts with a number of physiological partner proteins, including Glutaminase L, beta-Catenin, FAS, HTLV-1 Tax, HPV16 E6, Rhotekin and Kit 2.3. To identify the network of proteins that interact with GIP, a human fetal brain cDNA library was screened using a yeast two-hybrid assay with GIP as bait. We identified brain-specific angiogenesis inhibitor 2 (BAI2), a member of the adhesion-G protein-coupled receptors (GPCRs), as a new partner of GIP. BAI2 is expressed primarily in neurons, further expanding GIP cellular functions. The interaction between GIP and the carboxy-terminus of BAI2 was characterized using fluorescence, circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy assays. These biophysical analyses support the interaction identified in the yeast two-hybrid assay. This is the first study reporting BAI2 as an interaction partner of GIP. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipUSDA PECASE [2003-35302-12930]; NSFNational Science Foundation (NSF) [IBN-0628064]; USDAUnited States Department of Agriculture (USDA) [2011-65503-20030]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [DK082397]; NSERCNatural Sciences and Engineering Research Council of Canada [155268]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108T945]en_US
dc.description.sponsorshipThis research was financially supported by USDA PECASE Presidential Early Career Award for Scientists and Engineers award 2003-35302-12930, NSF Grant IBN-0628064, USDA Grant 2011-65503-20030 and NIH Grant DK082397 to Smita Mohanty, NSERC Grant 155268 to Melanie Dobson, and the Grant TUBITAK 108T945 to Zeki Topcu. We thank Dr. David L. Zoetewey for critical reading of this manuscript.en_US
dc.identifier.doi10.1016/j.bbrc.2011.07.029en_US
dc.identifier.endpage797en_US
dc.identifier.issn0006-291X
dc.identifier.issue4en_US
dc.identifier.pmid21787750en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage792en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2011.07.029
dc.identifier.urihttps://hdl.handle.net/11454/46154
dc.identifier.volume411en_US
dc.identifier.wosWOS:000294317300024en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlutaminase interacting proteinen_US
dc.subjectYeast-two-hybriden_US
dc.subjectBrain-specific angiogenesis inhibitor 2en_US
dc.titleIdentification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting proteinen_US
dc.typeArticleen_US

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