Stimulatory effect of 17ß-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

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dc.contributor.authorTaskiran D.
dc.contributor.authorEvren V.
dc.date.accessioned2019-10-27T08:33:46Z
dc.date.available2019-10-27T08:33:46Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractAdipose tissue-derived stem cells (ADSCs) are considered as a potential cell source for regenerative medicine and tissue engineering. Although ADSCs have greater proliferation capacity than bone marrow stem cells (BMSCs), lower differentiation ability of these cells limits their utility in experimental and clinical studies. The purpose of this study was to investigate whether 17ß-estradiol (E2) has a stimulatory effect on osteogenic differentiation potential of ADSCs in vitro. ADSCs were isolated from visceral adipose tissues of rats and treated with different concentrations of E 2 in osteogenic medium (OM) for 21 days. The differences in osteogenic differentiation potential of the cultures were assessed by von Kossa staining, measurement of alkaline phosphatase (ALP) activity and calcium levels. ADSCs cultured in OM supplemented with E2 showed greater bone-like nodule formation and mineral deposition in comparing with the cells grown in OM. In addition, ALP activity and calcium levels also were significantly higher in the cultures exposed to E2 than the cells treated only with OM (p < 0.005, n = 5). Our results suggest that E2 may stimulate the osteogenic differentiation of ADSCs and therefore, can be used as an inducing agent to improve the efficiency of these cells in in vitro and in vivo studies.en_US
dc.identifier.doi10.4149/gpb_2011_02_167en_US
dc.identifier.endpage174en_US
dc.identifier.issn0231-5882
dc.identifier.issue2en_US
dc.identifier.pmid21613672en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage167en_US
dc.identifier.urihttps://doi.org/10.4149/gpb_2011_02_167
dc.identifier.urihttps://hdl.handle.net/11454/26874
dc.identifier.volume30en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofGeneral Physiology and Biophysicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdipose tissueen_US
dc.subjectDifferentiationen_US
dc.subjectEstradiolen_US
dc.subjectOsteogenesisen_US
dc.subjectRaten_US
dc.subjectStem cellen_US
dc.titleStimulatory effect of 17ß-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cellsen_US
dc.typeArticleen_US

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