Investigation of bacterial and viral etiology in community acquired central nervous system infections with molecular methods [Toplum Kökenli Santral Sinir Sistemi Enfeksiyonlarinda Bakteriyel ve Viral Etiyolojinin Moleküler Yöntemlerle Degerlendirilmesi]

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dc.contributor.authorKahraman H.
dc.contributor.authorTünger A.
dc.contributor.authorŞenol S.
dc.contributor.authorGazi H.
dc.contributor.authorAvci M.
dc.contributor.authorÖrmen B.
dc.contributor.authorTürker N.
dc.contributor.authorAtalay S.
dc.contributor.authorKöse S.
dc.contributor.authorUlusoy S.
dc.contributor.authorTaşbakan M.I.
dc.contributor.authorSipahi O.R.
dc.contributor.authorYamazhan T.
dc.contributor.authorGülay Z.
dc.contributor.authorÇavuş S.A.
dc.contributor.authorPullukçu H.
dc.date.accessioned2019-10-27T08:03:49Z
dc.date.available2019-10-27T08:03:49Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this multicenter prospective cohort study, it was aimed to evaluate the bacterial and viral etiology in community-acquired central nervous system infections by standart bacteriological culture and multiplex polymerase chain reaction (PCR) methods. Patients hospitalized with central nervous system infections between April 2012 and February 2014 were enrolled in the study. Demographic and clinical information of the patients were collected prospectively. Cerebrospinal fluid (CSF) samples of the patients were examined by standart bacteriological culture methods, bacterial multiplex PCR (Seeplex meningitis-B ACE Detection (Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, Listeria monocytogenes, Group B streptococci) and viral multiplex PCR (Seeplex meningitis-VI ACE Detection kits herpes simplex virus-1(HSV1), herpes simplex virus-2(HSV2), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein Barr virus (EBV) and human herpes virus 6 (HHV6)) (Seeplex meningitis-V2 ACE Detection kit (enteroviruses)). Patients were classified as purulent meningitis, aseptic meningitis and encephalitis according to their clinical, CSF (leukocyte level, predominant cell type, protein and glucose (blood/CSF) levels) and cranial imaging results. Patients who were infected with a pathogen other than the detection of the kit or diagnosed as chronic meningitis and other diseases during the follow up, were excluded from the study. A total of 79 patients (28 feMale, 51 Male, aged 42.1 ±18.5) fulfilled the study inclusion criteria. A total of 46 patients were classified in purulent meningitis group whereas 33 were in aseptic meningitis/encephalitis group. Pathogens were detected by multiplex PCR in 41 patients. CSF cultures were positive in 10 (21.7%) patients (nine S.pneumoniae, one H.influenzae) and PCR were positive for 27 (58.6%) patients in purulent meningitis group. In this group one type of bacteria were detected in 18 patients (14 S.pneumoniae, two N.meningitidis, one H.influenzae, one Lmonocytogenes). Besides, it is noteworthy that multiple pathogens were detected such as bacteria-virus combination in eight patients and two different bacteria in one patient. In the aseptic meningitis/encephalitis group, pathogens were detected in 14 out of 33 patients; single type of viruses in 11 patients (seven enterovirus, two HSV1, one HSV2, one VZV) and two different viruses were determined in three patients. These data suggest that multiplex PCR methods may increase the isolation rate of pathogens in central nervous system infections. Existence of mixed pathogen growth is remarkable in our study. Further studies are needed for the clinical relevance of this result.en_US
dc.identifier.doi10.5578/mb.57358en_US
dc.identifier.endpage285en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid28929964en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage277en_US
dc.identifier.urihttps://doi.org/10.5578/mb.57358
dc.identifier.urihttps://hdl.handle.net/11454/25422
dc.identifier.volume51en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Societyen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcute bacterial meningitisen_US
dc.subjectEncephalitisen_US
dc.subjectMixed infectionen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectViral menengitisen_US
dc.titleInvestigation of bacterial and viral etiology in community acquired central nervous system infections with molecular methods [Toplum Kökenli Santral Sinir Sistemi Enfeksiyonlarinda Bakteriyel ve Viral Etiyolojinin Moleküler Yöntemlerle Degerlendirilmesi]en_US
dc.typeArticleen_US

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