Response Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensors

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dc.contributor.authorUrkut, Z.
dc.contributor.authorKara, P.
dc.contributor.authorGoksungur, Y.
dc.contributor.authorOzsoz, M.
dc.date.accessioned2019-10-27T21:35:33Z
dc.date.available2019-10-27T21:35:33Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractThe response surface methodology (RSM) is used for optimization of foodborne pathogen detection based on label free electrochemical nucleic acid biosensors. Listeria monocytogenes amplicons obtained from food samples are used as model case. The extent of hybridization is determined by using guanine oxidation signals obtained with Differential Pulse Voltammetry and Electrochemical Impedance Spectroscopy. RSM is used to investigate the effects of hybridization parameters including target and salt concentration and hybridization time on the biosensor selectivity. The ratio of electrochemical transductions after hybridization with complementary and noncomplementary targets is the response of the statistical analysis obtaining a detection limit of 267 pM.en_US
dc.identifier.doi10.1002/elan.201100310
dc.identifier.endpage2676en_US
dc.identifier.issn1040-0397
dc.identifier.issn1040-0397en_US
dc.identifier.issue11en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2668en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201100310
dc.identifier.urihttps://hdl.handle.net/11454/45885
dc.identifier.volume23en_US
dc.identifier.wosWOS:000297311500025en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNucleic acid biosensoren_US
dc.subjectVoltammetryen_US
dc.subjectElectrochemical impedance spectroscopy (EIS)en_US
dc.subjectListeria monocytogenesen_US
dc.subjectResponse surface methodology (RSM)en_US
dc.titleResponse Surface Methodology for Optimization of Food Borne Pathogen Detection in Real Samples Based on Label Free Electrochemical Nucleic Acid Biosensorsen_US
dc.typeArticleen_US

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