Mass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolica

Basit Öğe Kaydı
dc.contributor.authorGocmen, Bayram
dc.contributor.authorHeiss, Paul
dc.contributor.authorPetras, Daniel
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorSuessmuth, Roderich D.
dc.date.accessioned2019-10-27T22:59:13Z
dc.date.available2019-10-27T22:59:13Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThis contribution reports on the first characterization of the venom proteome and the bioactivity screening of Vipera anatolica, the Anatolian Meadow Viper. The crude venom as well as an isolated dimeric disintegrin showed remarkable cytotoxic activity against glioblastoma cells. Due to the rare occurrence and the small size of this species only little amount of venom was available, which was profiled by means of a combination of bottom-up and top-down mass spectrometry. From this analysis we identified snake venom metalloproteases, cysteine-rich secretory protein isoforms, a metalloprotease inhibitor, several type A2 phospholipases, disintegrins, a snake venom serine protease, a C-type lectin and a Kunitz-type protease inhibitor. Furthermore, we detected several isoforms of above mentioned proteins as well as previously unknown proteins, indicating an extensive complexity of the venom which would have remained undetected with conventional venomic approaches. (C) 2015 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG); Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T338]; Cluster of Excellence Unifying Concepts in Catalysis (UniCat)German Research Foundation (DFG)en_US
dc.description.sponsorshipThis study was supported by the Deutsche Forschungsgemeinschaft (DFG), Cluster of Excellence Unifying Concepts in Catalysis (UniCat) and by the Scientific and Technical Research Council of Turkey (TUBITAK) under Grant 111T338. We thank Dr. Rashed Al Toma and Eric van Herwerden (TU Berlin) for helpful discussion on the manuscript as well as Mert Karis M. Anil Oguz and Volkan Eroglu (Ege University, Izmir) for field assistances. We also thank to AREL (Ege University School of Medicine Research and Education Laboratory) for the permission to use their laboratory.en_US
dc.identifier.doi10.1016/j.toxicon.2015.09.013en_US
dc.identifier.endpage174en_US
dc.identifier.issn0041-0101
dc.identifier.pmid26385313en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage163en_US
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2015.09.013
dc.identifier.urihttps://hdl.handle.net/11454/51748
dc.identifier.volume107en_US
dc.identifier.wosWOS:000366539600002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofToxiconen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectViperidaeen_US
dc.subjectAnatolian Meadow Viperen_US
dc.subjectVipera anatolicaen_US
dc.subjectSnake venomicsen_US
dc.subjectTop-down venomicsen_US
dc.subjectCytotoxicityen_US
dc.subjectBioactivity screeningen_US
dc.titleMass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolicaen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu