Production of antimicrobial films by incorporation of partially purified lysozyme into biodegradable films of crude exopolysaccharides obtained from Aureobasidium pullulans fermentation

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dc.contributor.authorKandemir, N
dc.contributor.authorYemenicioglu, A
dc.contributor.authorMecitoglu, I
dc.contributor.authorElmaci, ZS
dc.contributor.authorArslanoglu, A
dc.contributor.authorGoksungur, Y
dc.contributor.authorBaysal, T
dc.date.accessioned2019-10-27T19:22:55Z
dc.date.available2019-10-27T19:22:55Z
dc.date.issued2005
dc.departmentEge Üniversitesien_US
dc.description.abstractAntimicrobial films were produced by incorporating partially purified lysozyme into films of crude exopolysaccharides (59 % pullulan) obtained from Aureobasidium pullulans fermentation. After film making, the films containing lysozyme at 100, 260, 520 and 780 mu g/cm(2) showed 23 to 70 % of their expected enzyme activities. The highest recovery of enzyme activity (65-70 %) after the film making was obtained in films prepared by incorporating lysozyme at 260 mu g/cm(2) (1409 U/cm(2)). The incorporation of disodium EDTA(.)2H(2)O and sucrose did not affect the initial lysozyme activity of the films significantly. With or without the presence of disodium EDTA(.)2H(2)O at 52 or 520 mu g/cm(2), lysozyme activity showed sufficient stability in the films during 21 days of cold storage. However, the presence of sucrose at 10 mg/cm(2) in the films caused the destabilization of part of enzyme activity (almost 35 %) at the end of storage. The combinational incorporation of lysozyme at 780 pg/cm(2) (4227 U/cm(2)) and disodium EDTA(.)2H(2)O at 520 mu g/cm(2) gave antimicrobial films effective on Escherichia coli. However, in the studied lysozyme concentration range the films did not show any antimicrobial activity against Lactobacillus plantarum. This study clearly showed that the partially purified lysozyme and crude exopolysaccharides from Aureobasidium pullulans may be used to obtain antimicrobial films to increase the safety of foods.en_US
dc.identifier.endpage350en_US
dc.identifier.issn1330-9862
dc.identifier.issn1334-2606
dc.identifier.issn1330-9862en_US
dc.identifier.issn1334-2606en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage343en_US
dc.identifier.urihttps://hdl.handle.net/11454/39135
dc.identifier.volume43en_US
dc.identifier.wosWOS:000234084600005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFaculty Food Technology Biotechnologyen_US
dc.relation.ispartofFood Technology and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectlysozymeen_US
dc.subjectexopolysaccharidesen_US
dc.subjectpullulanen_US
dc.subjectAureobasidium pullulansen_US
dc.subjectantimicrobial filmsen_US
dc.titleProduction of antimicrobial films by incorporation of partially purified lysozyme into biodegradable films of crude exopolysaccharides obtained from Aureobasidium pullulans fermentationen_US
dc.typeArticleen_US

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